Abstract
The Sir1 protein plays a key role in establishing a silent chromatin structure at the cryptic mating-type loci HMR and HML in Saccharomyces cerevisiae by interacting with the bromo-adjacent homology (BAH) domain of the Orc1p subunit of the origin recognition complex (ORC). Here, we present the high-resolution crystal structures of the ORC interaction region (OIR) of Sir1p and that of the complex formed between the OIR and BAH domains. Amino acids within the OIR previously shown to be required for a Sir1p/ORC interaction are presented on a conserved, convex surface that forms a complementary interface with a concave region of the Orc1 BAH domain that is critical for transcriptional silencing. The OIR/BAH interaction surface comprises a network of hydrophobic and polar/ionic interactions between discrete structural modules in each protein and involves several residues that were not implicated in previous studies. These data provide important structural insights into a protein-protein interaction critical for the formation of a specialized chromatin domain within eukaryotic chromosomes.
MeSH Terms
Amino Acid Sequence
Amino Acids/metabolism
Conserved Sequence/genetics
Crystallography
DNA-Binding Proteins/metabolism
Gene Silencing
Models, Molecular
Molecular Sequence Data
Origin Recognition Complex
Protein Binding
Protein Structure, Tertiary
Saccharomyces cerevisiae/genetics,metabolism
Saccharomyces cerevisiae Proteins
Silent Information Regulator Proteins, Saccharomyces cerevisiae/chemistry,genetics,metabolism
Chemicals
Amino Acids
DNA-Binding Proteins
ORC1 protein, S cerevisiae
Origin Recognition Complex
SIR1 protein, S cerevisiae
Saccharomyces cerevisiae Proteins
Silent Information Regulator Proteins, Saccharomyces cerevisiae
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hou Zhonggang
Department of Biomolecular Chemistry, University of Wisconsin Medical School, Medical Sciences Center, 1300 University Avenue, Madison, WI 53706-1532, USA.
Bernstein Douglas A
Fox Catherine A
Keck James L
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