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PMID: 1592832 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Purification and characterization of the acyl carrier protein of the Streptomyces glaucescens tetracenomycin C polyketide synthase.

Journal of bacteriology ·Vol. 174 ·No. 11 ·1992-06-00 ·Pages 3818-21

Shen B, Summers RG, Gramajo H, Bibb MJ, Hutchinson CR

Abstract

The acyl carrier protein (ACP) of the tetracenomycin C polyketide synthase, encoded by the tcmM gene, has been expressed in both Streptomyces glaucescens and Escherichia coli and purified to homogeneity. Expression of the tcmM gene in E. coli results mainly in the TcmM apo-ACP, whereas expression in S. glaucescens yields solely the holo-ACP. The purified holo-TcmM is active in a malonyl coenzyme A:ACP transacylase assay and is labeled by radioactive beta-alanine, confirming that it carries a 4'-phosphopantetheine prosthetic group.

Related Genes
tcmM
MeSH Terms
Acyl Carrier Protein/biosynthesis,isolation & purification Amino Acid Sequence Apoproteins/biosynthesis Base Sequence Escherichia coli/genetics Molecular Sequence Data Multienzyme Complexes/biosynthesis Naphthacenes/metabolism Sequence Homology, Nucleic Acid Streptomyces/enzymology,genetics
Chemicals
Acyl Carrier Protein Apoproteins Multienzyme Complexes Naphthacenes tetracenomycin C
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Shen B
School of Pharmacy, University of Wisconsin, Madison 53706.
Summers R G
Gramajo H
Bibb M J
Hutchinson C R
References (19)
19 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.174.11.3818-3821.1992
Published
1992-06-00
Pages
3818-21
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC206074
Subset
IM
Grants
NCI NIH HHS · CA 35381 · United States
External Links
PubMed source DOI: 10.1128/jb.174.11.3818-3821.1992 PMC Full Text View journal details
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