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PMID: 1592829 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Proline biosynthesis in Saccharomyces cerevisiae: analysis of the PRO3 gene, which encodes delta 1-pyrroline-5-carboxylate reductase.

Journal of bacteriology ·Vol. 174 ·No. 11 ·1992-06-00 ·Pages 3782-8

Brandriss MC, Falvey DA

Abstract

The PRO3 gene of Saccharomyces cerevisiae encodes the 286-amino-acid protein delta 1-pyrroline-5-carboxylate reductase [L-proline:NAD(P+) 5-oxidoreductase; EC 1.5.1.2], which catalyzes the final step in proline biosynthesis. The protein has substantial similarity to the pyrroline carboxylate reductases of diverse bacterial species, soybean, and humans. Using RNA hybridization and measurements of enzyme activity, we have determined that the expression of the PRO3 gene appears to be constitutive. It is not repressed by the pathway end product (proline), induced by the initial substrate (glutamate), or regulated by the general control system. Its expression is not detectably altered when cells are grown in a wide range of nitrogen sources or when glycerol and ethanol replace glucose as the carbon source. The possibility that this enzyme has other functions in addition to proline biosynthesis is discussed.

Related Genes
PRO3
MeSH Terms
Amino Acid Sequence Amino Acids/pharmacology Amitrole/pharmacology Base Sequence Culture Media/pharmacology Gene Expression Regulation, Fungal/drug effects,genetics Molecular Sequence Data Proline/biosynthesis,pharmacology Pyrroline Carboxylate Reductases/biosynthesis,genetics Saccharomyces cerevisiae/genetics Sequence Homology, Nucleic Acid beta-Galactosidase/biosynthesis
Chemicals
Amino Acids Culture Media Proline Pyrroline Carboxylate Reductases delta-1-pyrroline-5-carboxylate reductase beta-Galactosidase Amitrole
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Brandriss M C
Department of Microbiology and Molecular Genetics, UMDNJ-New Jersey Medical School, Newark 07103.
Falvey D A
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41 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.174.11.3782-3788.1992
Published
1992-06-00
Pages
3782-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC206069
Subset
IM
Grants
NIGMS NIH HHS · GM40751 · United States
Databases
GENBANK
M57886, M79367, M79368, M79369, M79370, M79371, M79372, M84973, M84974, M84980
Corrections
ErratumIn
PMID: 1352771
External Links
PubMed source DOI: 10.1128/jb.174.11.3782-3788.1992 PMC Full Text View journal details
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