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PMID: 15923633 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Combination of hTERT and bmi-1, E6, or E7 induces prolongation of the life span of bone marrow stromal cells from an elderly donor without affecting their neurogenic potential.

Molecular and cellular biology ·Vol. 25 ·No. 12 ·2005-06-00 ·Pages 5183-95

Mori T, Kiyono T, Imabayashi H, Takeda Y, Tsuchiya K, Miyoshi S, Makino H, Matsumoto K, Saito H, Ogawa S, Sakamoto M, Hata J, Umezawa A

Abstract

Murine bone marrow stromal cells differentiate not only into mesodermal derivatives, such as osteocytes, chondrocytes, adipocytes, skeletal myocytes, and cardiomyocytes, but also into neuroectodermal cells in vitro. Human bone marrow stromal cells are easy to isolate but difficult to study because of their limited life span. To overcome this problem, we attempted to prolong the life span of bone marrow stromal cells and investigated whether bone marrow stromal cells modified with bmi-1, hTERT, E6, and E7 retained their differentiated capability, or multipotency. In this study, we demonstrated that the life span of bone marrow stromal cells derived from a 91-year-old donor could be extended and that the stromal cells with an extended life span differentiated into neuronal cells in vitro. We examined the neuronally differentiated cells morphologically, physiologically, and biologically and compared the gene profiles of undifferentiated and differentiated cells. The neuronally differentiated cells exhibited characteristics similar to those of midbrain neuronal progenitors. Thus, the results of this study support the possible use of autologous-cell graft systems to treat central nervous system diseases in geriatric patients.

MeSH Terms
Aged Aged, 80 and over Animals Bone Marrow Cells/cytology,physiology Calcium/metabolism Cell Cycle Proteins/genetics,metabolism Cell Differentiation/physiology Cell Lineage Cells, Cultured DNA-Binding Proteins Female Gene Expression Profiling Gene Expression Regulation Humans Karyotyping Mice Multipotent Stem Cells/cytology,physiology Neurons/cytology,physiology Nuclear Proteins/genetics,metabolism Oligonucleotide Array Sequence Analysis Oncogene Proteins, Viral/genetics,metabolism Polycomb Repressive Complex 1 Proto-Oncogene Proteins/genetics,metabolism Repressor Proteins/genetics,metabolism Stromal Cells/cytology,physiology Telomerase/genetics,metabolism Telomere/metabolism
Chemicals
Bmi1 protein, mouse Cell Cycle Proteins DNA-Binding Proteins Nuclear Proteins Oncogene Proteins, Viral Proto-Oncogene Proteins Repressor Proteins Polycomb Repressive Complex 1 Telomerase Calcium
Authors & Affiliations
13 authors, click to expand affiliations / ORCID
Mori Taisuke
Department of Reproductive Biology and Pathology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya-ku, Tokyo 157-8535, Japan.
Kiyono Tohru
Imabayashi Hideaki
Takeda Yukiji
Tsuchiya Kohei
Miyoshi Shunichirou
Makino Hatsune
Matsumoto Kenji
Saito Hirohisa
Ogawa Satoshi
Sakamoto Michiie
Hata Jun-Ichi
Umezawa Akihiro
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.25.12.5183-5195.2005
Published
2005-06-00
Pages
5183-95
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC1140572
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.25.12.5183-5195.2005 PMC Full Text View journal details
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