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PMID: 15923332 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The AtRAD51C gene is required for normal meiotic chromosome synapsis and double-stranded break repair in Arabidopsis.

Plant physiology ·Vol. 138 ·No. 2 ·2005-06-00 ·Pages 965-76

Li W, Yang X, Lin Z, Timofejeva L, Xiao R, Makaroff CA, Ma H

Abstract

Meiotic prophase I is a complex process involving homologous chromosome (homolog) pairing, synapsis, and recombination. The budding yeast (Saccharomyces cerevisiae) RAD51 gene is known to be important for recombination and DNA repair in the mitotic cell cycle. In addition, RAD51 is required for meiosis and its Arabidopsis (Arabidopsis thaliana) ortholog is important for normal meiotic homolog pairing, synapsis, and repair of double-stranded breaks. In vertebrate cell cultures, the RAD51 paralog RAD51C is also important for mitotic homologous recombination and maintenance of genome integrity. However, the function of RAD51C in meiosis is not well understood. Here we describe the identification and analysis of a mutation in the Arabidopsis RAD51C ortholog, AtRAD51C. Although the atrad51c-1 mutant has normal vegetative and flower development and has no detectable abnormality in mitosis, it is completely male and female sterile. During early meiosis, homologous chromosomes in atrad51c-1 fail to undergo synapsis and become severely fragmented. In addition, analysis of the atrad51c-1 atspo11-1 double mutant showed that fragmentation was nearly completely suppressed by the atspo11-1 mutation, indicating that the fragmentation largely represents a defect in processing double-stranded breaks generated by AtSPO11-1. Fluorescence in situ hybridization experiments suggest that homolog juxtaposition might also be abnormal in atrad51c-1 meiocytes. These results demonstrate that AtRAD51C is essential for normal meiosis and is probably required for homologous synapsis.

MeSH Terms
Arabidopsis/cytology,genetics Arabidopsis Proteins/genetics,physiology Chromosome Pairing/physiology Chromosomes, Plant DNA Repair/physiology Meiosis/genetics Mutation Phenotype
Chemicals
Arabidopsis Proteins AtRAD51C protein, Arabidopsis
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Li Wuxing
Department of Biology and the Huck Institutes of the Life Sciences , the Pennsylvania State University, University Park, Pennsylvania 16802, USA.
Yang Xiaohui
Lin Zhenguo
Timofejeva Ljudmilla
Xiao Rong
Makaroff Christopher A
Ma Hong
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.104.058347
Published
2005-06-00
Epub
2005-00-27
Pages
965-76
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC1150411
Subset
IM
Grants
NIGMS NIH HHS · R01 GM63871-01 · United States
External Links
PubMed source DOI: 10.1104/pp.104.058347 PMC Full Text View journal details
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