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PMID: 1589796 Published · ppublish English Comment Comparative Study Journal Article

Molecular epidemiology of HIV transmission in a dental practice.

Science (New York, N.Y.) ·Vol. 256 ·No. 5060 ·1992-05-22 ·Pages 1165-71

Ou CY, Ciesielski CA, Myers G, Bandea CI, Luo CC, Korber BT, Mullins JI, Schochetman G, Berkelman RL, Economou AN

Abstract

Human immunodeficiency virus type 1 (HIV-1) transmission from infected patients to health-care workers has been well documented, but transmission from an infected health-care worker to a patient has not been reported. After identification of an acquired immunodeficiency syndrome (AIDS) patient who had no known risk factors for HIV infection but who had undergone an invasive procedure performed by a dentist with AIDS, six other patients of this dentist were found to be HIV-infected. Molecular biologic studies were conducted to complement the epidemiologic investigation. Portions of the HIV proviral envelope gene from each of the seven patients, the dentist, and 35 HIV-infected persons from the local geographic area were amplified by polymerase chain reaction and sequenced. Three separate comparative genetic analyses--genetic distance measurements, phylogenetic tree analysis, and amino acid signature pattern analysis--showed that the viruses from the dentist and five dental patients were closely related. These data, together with the epidemiologic investigation, indicated that these patients became infected with HIV while receiving care from a dentist with AIDS.

MeSH Terms

Acquired Immunodeficiency Syndrome/blood,microbiology,transmission Amino Acid Sequence Base Sequence DNA, Viral/blood,genetics,isolation & purification Dentistry Female Florida Genetic Variation HIV Infections/microbiology,transmission HIV-1/genetics,isolation & purification Humans Male Molecular Sequence Data Monocytes/physiology Oligodeoxyribonucleotides Patients Phylogeny Sequence Homology, Nucleic Acid Viral Envelope Proteins/genetics

Chemicals

DNA, Viral Oligodeoxyribonucleotides Viral Envelope Proteins

Authors & Affiliations

10 authors, click to expand affiliations / ORCID

Ou C Y

Division of HIV/AIDS, Centers for Disease Control, Atlanta, GA 30333.

Ciesielski C A

Myers G

Bandea C I

Luo C C

Korber B T

Mullins J I

Schochetman G

Berkelman R L

Economou A N

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.256.5060.1165

Published

1992-05-22

Pages

1165-71

Language

English

Region

United States

NLM ID

0404511

Subset

Databases

GENBANK

M90847, M90848, M90849, M90850, M90851, M90852, M90853, M90854, M90855, M90856, M90857, M90858, M90859, M90860, M90861, M90862, M90863, M90864, M90865, M90866, M90867, M90868, M90869, M90870, M90871, M90872, M90873, M90874, M90875, M90876

Corrections

CommentOn

PMID: 1589795

External Links

PubMed source DOI: 10.1126/science.256.5060.1165 View journal details

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Molecular epidemiology of HIV transmission in a dental practice.

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