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PMID: 15886885 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Review

The mammary gland "side population": a putative stem/progenitor cell marker?

Journal of mammary gland biology and neoplasia ·Vol. 10 ·No. 1 ·2005-01-00 ·Pages 37-47

Smalley MJ, Clarke RB

Abstract

Hematopoietic Stem Cells have been isolated by their ability to pump out Hoechst 33342 dye and form a distinct population definable by flow cytometry--the Side Population (SP). The membrane pump Bcrp has been identified as the molecular determinant of the SP phenotype. An SP population with Bcrp activity has been defined in a number of tissues, including mouse mammary and human breast epithelium, and it has been proposed that the SP phenotype is a universal stem cell marker. Studies of mouse and human breast SP suggest that the population is undifferentiated but capable of differentiating into epithelial structures of both luminal and myoepithelial lineages both in vitro and in vivo. However, evidence that the SP is enriched for stem cells is, at the moment, only correlative, and there are potentially confounding technical issues. We still await formal proof that the SP contains a stem cell population.

MeSH Terms
ATP-Binding Cassette Transporters/genetics,metabolism Animals Biomarkers/analysis Breast Neoplasms/metabolism,pathology Humans Mammary Glands, Animal/cytology,metabolism Mammary Glands, Human/cytology,metabolism Stem Cells/cytology,metabolism
Chemicals
ATP-Binding Cassette Transporters Biomarkers
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Smalley Matthew J
The Breakthrough Breast Cancer Centre, The Institute of Cancer Research, London, United Kingdom.
Clarke Robert B
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Article Info
Journal
Journal of mammary gland biology and neoplasia
Abbr.
J Mammary Gland Biol Neoplasia
ISSN
1083-3021
DOI
10.1007/s10911-005-2539-0
Published
2005-01-00
Pages
37-47
Language
English
Region
United States
NLM ID
9601804
Subset
IM
Grants
Breast Cancer Now · 2004NOV42 · United Kingdom
Breast Cancer Now · BREAST CANCER NOW RESEARCH CENTRE · United Kingdom
External Links
PubMed source DOI: 10.1007/s10911-005-2539-0 View journal details
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