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PMID: 15872023 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

HIV-1-specific IFN-gamma/IL-2-secreting CD8 T cells support CD4-independent proliferation of HIV-1-specific CD8 T cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 102 ·No. 20 ·2005-05-17 ·Pages 7239-44

Zimmerli SC, Harari A, Cellerai C, Vallelian F, Bart PA, Pantaleo G

Abstract

Functional and phenotypic characterization of virus-specific CD8 T cells against cytomegalovirus, Epstein-Barr virus, influenza (flu), and HIV-1 were performed on the basis of the ability of CD8 T cells to secrete IFN-gamma and IL-2, to proliferate, and to express CD45RA and CCR7. Two functional distinct populations of CD8 T cells were identified: (i) dual IFN-gamma/IL-2-secreting cells and (ii) single IFN-gamma-secreting cells. Virus-specific IFN-gamma/IL-2-secreting CD8 T cells were CD45RA-CCR7-, whereas single IFN-gamma CD8 T cells were either CD45RA-CCR7- or CD45RA+CCR7-. The proportion of virus-specific IFN-gamma/IL-2-secreting CD8 T cells correlated with that of proliferating CD8 T cells, and the loss of HIV-1-specific IL-2-secreting CD8 T cells was associated with that of HIV-1-specific CD8 T cell proliferation. Substantial proliferation of virus-specific CD8 T cells (including HIV-1-specific CD8 T cells) was also observed in CD4 T cell-depleted populations or after stimulation with MHC class I tetramer-peptide complexes. IL-2 was the factor responsible for the CD4-independent CD8 T cell proliferation. These results indicate that IFN-gamma/IL-2-secreting CD8 T cells may promote antigen-specific proliferation of CD8 T cells even in the absence of helper CD4 T cells.

MeSH Terms
CD4 Antigens/immunology CD8-Positive T-Lymphocytes/immunology,metabolism,virology Cell Proliferation Cytomegalovirus/immunology Flow Cytometry HIV Infections/immunology HIV-1 Herpesvirus 4, Human/immunology Humans Interferon-gamma/metabolism Interleukin-2/metabolism Leukocyte Common Antigens/metabolism Orthomyxoviridae/immunology Receptors, CCR7 Receptors, Chemokine/metabolism
Chemicals
CCR7 protein, human CD4 Antigens Interleukin-2 Receptors, CCR7 Receptors, Chemokine Interferon-gamma Leukocyte Common Antigens
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Zimmerli Simone C
Department of Medicine, Division of Immunology and Allergy, Laboratory of AIDS Immunopathogenesis, Centre Hospitalier Universitaire Vaudois, University of Lausanne, 1011 Lausanne, Switzerland.
Harari Alexandre
Cellerai Cristina
Vallelian Florence
Bart Pierre-Alexandre
Pantaleo Giuseppe
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0502393102
Published
2005-05-17
Epub
2005-00-04
Pages
7239-44
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC1088398
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.0502393102 PMC Full Text View journal details
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