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PMID: 15870363 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Changes in nitrogen-fixing and ammonia-oxidizing bacterial communities in soil of a mixed conifer forest after wildfire.

Applied and environmental microbiology ·Vol. 71 ·No. 5 ·2005-05-00 ·Pages 2713-22

Yeager CM, Northup DE, Grow CC, Barns SM, Kuske CR

Abstract

This study was undertaken to examine the effects of forest fire on two important groups of N-cycling bacteria in soil, the nitrogen-fixing and ammonia-oxidizing bacteria. Sequence and terminal restriction fragment length polymorphism (T-RFLP) analysis of nifH and amoA PCR amplicons was performed on DNA samples from unburned, moderately burned, and severely burned soils of a mixed conifer forest. PCR results indicated that the soil biomass and proportion of nitrogen-fixing and ammonia-oxidizing species was less in soil from the fire-impacted sites than from the unburned sites. The number of dominant nifH sequence types was greater in fire-impacted soils, and nifH sequences that were most closely related to those from the spore-forming taxa Clostridium and Paenibacillus were more abundant in the burned soils. In T-RFLP patterns of the ammonia-oxidizing community, terminal restriction fragments (TRFs) representing amoA cluster 1, 2, or 4 Nitrosospira spp. were dominant (80 to 90%) in unburned soils, while TRFs representing amoA cluster 3A Nitrosospira spp. dominated (65 to 95%) in fire-impacted soils. The dominance of amoA cluster 3A Nitrosospira spp. sequence types was positively correlated with soil pH (5.6 to 7.5) and NH(3)-N levels (0.002 to 0.976 ppm), both of which were higher in burned soils. The decreased microbial biomass and shift in nitrogen-fixing and ammonia-oxidizing communities were still evident in fire-impacted soils collected 14 months after the fire.

MeSH Terms
Ammonia/metabolism Bacteria/isolation & purification,metabolism Base Sequence Fires Molecular Sequence Data Nitrogen Fixation Oxidation-Reduction Oxidoreductases/genetics Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Soil/analysis Soil Microbiology Trees
Chemicals
Soil Ammonia Oxidoreductases nitrogenase reductase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Yeager Chris M
Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.
Northup Diana E
Grow Christy C
Barns Susan M
Kuske Cheryl R
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/AEM.71.5.2713-2722.2005
Published
2005-05-00
Pages
2713-22
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC1087562
Subset
IM
Databases
GENBANK
AY819559, AY819560, AY819561, AY819562, AY819563, AY819564, AY819565, AY819566, AY819567, AY819568, AY819569, AY819570, AY819571, AY819572, AY819573, AY819574, AY819575, AY819576, AY819577, AY819578, AY819579, AY819580, AY819581, AY819582, AY819583, AY819584, AY819585, AY819586, AY819587, AY819588, AY819589, AY819590, AY819591, AY819592, AY819593, AY819594, AY819595, AY819596, AY819597, AY819598, AY819599, AY819600, AY819601, AY819602, AY819603, AY819604, AY819605, AY819606, AY819607, AY819608, AY819609, AY819610, AY819611, AY819612, AY819613, AY819614, AY819615, AY819616, AY819617, AY819618, AY819619, AY819620, AY819621, AY819622, AY819623, AY819624, AY819625, AY819626
External Links
PubMed source DOI: 10.1128/AEM.71.5.2713-2722.2005 PMC Full Text View journal details
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