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PMID: 15862294 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Roles of Mg2+ in TPP-dependent riboswitch.

FEBS letters ·Vol. 579 ·No. 12 ·2005-05-09 ·Pages 2583-8

Yamauchi T, Miyoshi D, Kubodera T, Nishimura A, Nakai S, Sugimoto N

Abstract

We quantified the effect of Mg(2+) on thiamine pyrophosphate (TPP) binding to TPP-dependent thiA riboswitch RNA. The association constant of TPP binding to the riboswitch at 20 degrees C increased from 1.2 x 10(6) to 50 x 10(6) M(-1) as the Mg(2+) concentration increased from 0 to 1 mM. Furthermore, circular dichroic spectra under various conditions showed that 1 mM Mg(2+) induced a local structural change of the riboswitch, which might be pivotal for TPP binding. These results indicate that a physiological concentration of Mg(2+) can regulate TPP binding to the thiA riboswitch.

MeSH Terms

5' Untranslated Regions Adenosine/metabolism Base Sequence Binding Sites Circular Dichroism Conserved Sequence Dose-Response Relationship, Drug Electrophoresis, Polyacrylamide Gel Gene Expression Regulation Kinetics Magnesium/metabolism,pharmacology,physiology Models, Molecular Molecular Sequence Data Protein Binding RNA/chemistry,classification,genetics,metabolism Sequence Analysis, RNA Sequence Homology, Nucleic Acid Temperature Thiamine Pyrophosphate/metabolism Titrimetry Transcription, Genetic

Chemicals

5' Untranslated Regions RNA Magnesium Adenosine Thiamine Pyrophosphate

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Yamauchi Takahiro

Frontier Institute for Biomolecular Engineering, Research (FIBER), Konan University, Higashinada-ku, Kobe, Japan.

Miyoshi Daisuke

Kubodera Takafumi

Nishimura Akira

Nakai Susumu

Sugimoto Naoki

Article Info

Journal

FEBS letters

Abbr.

FEBS Lett

ISSN

0014-5793

DOI

10.1016/j.febslet.2005.03.074

Published

2005-05-09

Epub

2005-00-08

Pages

2583-8

Language

English

Region

England

NLM ID

0155157

Subset

External Links

PubMed source DOI: 10.1016/j.febslet.2005.03.074 View journal details

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