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PMID: 15845531 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Conversion of Staphylococcus epidermidis strains from commensal to invasive by expression of the ica locus encoding production of biofilm exopolysaccharide.

Infection and immunity ·Vol. 73 ·No. 5 ·2005-05-00 ·Pages 3188-91

Li H, Xu L, Wang J, Wen Y, Vuong C, Otto M, Gao Q

Abstract

To test if biofilm formation in Staphylococcus epidermidis is dependent on the polysaccharide intercellular adhesin, whose biosynthesis is driven by the ica locus, a plasmid containing the ica locus was transferred to three ica-negative strains. Using in vitro biofilm assays and a rat central venous catheter infection model, we confirmed the importance of the ica locus for biofilm production and pathogenesis of S. epidermidis.

MeSH Terms
Animals Bacterial Adhesion Bacterial Proteins/genetics,metabolism Biofilms/growth & development Catheterization, Central Venous/adverse effects Gene Expression Regulation, Bacterial Humans Microscopy, Electron, Scanning Molecular Sequence Data Multigene Family Plasmids Polysaccharides, Bacterial/genetics,metabolism Prosthesis-Related Infections/microbiology Rats Staphylococcus epidermidis/genetics,growth & development,metabolism,pathogenicity Streptococcal Infections/microbiology Virulence
Chemicals
Bacterial Proteins Polysaccharides, Bacterial polysaccharide intercellular adhesin
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Li Hualin
Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai, 200032, PR China.
Xu Lin
Wang Jianping
Wen Yumei
Vuong Cuong
Otto Michael
Gao Qian
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/IAI.73.5.3188-3191.2005
Published
2005-05-00
Pages
3188-91
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC1087319
Subset
IM
Databases
GENBANK
AY382582
External Links
PubMed source DOI: 10.1128/IAI.73.5.3188-3191.2005 PMC Full Text View journal details
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