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PMID: 15838050 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Dynamics of FtsZ assembly during sporulation in Streptomyces coelicolor A3(2).

Journal of bacteriology ·Vol. 187 ·No. 9 ·2005-05-00 ·Pages 3227-37

Grantcharova N, Lustig U, Flärdh K

Abstract

FtsZ, the bacterial tubulin homologue, is the main player in at least two distinct processes of cell division during the development of Streptomyces coelicolor A3(2). It forms cytokinetic rings and is required for the formation of both the widely spaced hyphal cross walls in the substrate mycelium and the specialized septation that converts sporogenic aerial hyphae into spores. The latter developmentally controlled septation involves the coordinated assembly of large numbers of FtsZ rings in each sporulating hyphal cell. We used an FtsZ-enhanced green fluorescent protein (EGFP) translational fusion to visualize the progression of FtsZ ring assembly in vivo during sporulation of aerial hyphae. This revealed that the regular placement of multiple FtsZ rings and initiation of cytokinesis was preceded by a protracted phase during which spiral-shaped FtsZ intermediates were detected along the length of the aerial hyphal cell. Time course experiments indicated that they were remodeled and gradually replaced by regularly spaced FtsZ rings. Such spiral-shaped filaments could also be detected with immunofluorescence microscopy using an antiserum against FtsZ. Based on our observations, we propose a model for the progression of Z-ring assembly during sporulation of S. coelicolor. Furthermore, mutants lacking the developmental regulatory genes whiA, whiB, whiG, whiH, and whiI were investigated. They failed in up-regulation of the expression of FtsZ-EGFP in aerial hyphae, which is consistent with the known effects of these genes on ftsZ transcription.

MeSH Terms
Bacterial Proteins/analysis,genetics,metabolism,physiology Biopolymers/metabolism Cytoskeletal Proteins/analysis,metabolism DNA-Binding Proteins/genetics,physiology Green Fluorescent Proteins/genetics Microscopy, Fluorescence Mutation Recombinant Fusion Proteins/genetics Repressor Proteins/genetics,physiology Sigma Factor/genetics,physiology Spores, Bacterial/genetics,growth & development Streptomyces coelicolor/genetics,physiology Transcription Factors/genetics,physiology
Chemicals
Bacterial Proteins Biopolymers Cytoskeletal Proteins DNA-Binding Proteins FtsZ protein, Bacteria Recombinant Fusion Proteins Repressor Proteins Sigma Factor Transcription Factors WhiA protein, Streptomyces coelicolor whiB protein, Streptomyces whiH protein, Streptomyces whiG protein, Streptomyces coelicolor Green Fluorescent Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Grantcharova Nina
Dept. of Cell and Organism Biology, Uppsala University, SE-751 24 Uppsala, Sweden.
Lustig Ulrika
Flärdh Klas
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.187.9.3227-3237.2005
Published
2005-05-00
Pages
3227-37
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC1082811
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.187.9.3227-3237.2005 PMC Full Text View journal details
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