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PMID: 15838041 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, P.H.S.

Staphylococcus intermedius produces a functional agr autoinducing peptide containing a cyclic lactone.

Journal of bacteriology ·Vol. 187 ·No. 9 ·2005-05-00 ·Pages 3139-50

Ji G, Pei W, Zhang L, Qiu R, Lin J, Benito Y, Lina G, Novick RP

Abstract

The agr system is a global regulator of accessory functions in staphylococci, including genes encoding exoproteins involved in virulence. The agr locus contains a two-component signal transduction module that is activated by an autoinducing peptide (AIP) encoded within the agr locus and is conserved throughout the genus. The AIP has an unusual partially cyclic structure that is essential for function and that, in all but one case, involves an internal thiolactone bond between a conserved cysteine and the C-terminal carboxyl group. The exceptional case is a strain of Staphylococcus intermedius that has a serine in place of the conserved cysteine. We demonstrate here that the S. intermedius AIP is processed by the S. intermedius AgrB protein to generate a cyclic lactone, that it is an autoinducer as well as a cross-inhibitor, and that all of five other S. intermedius strains examined also produce serine-containing AIPs.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/chemistry,genetics,physiology DNA, Bacterial/isolation & purification Gene Expression Regulation, Bacterial Hemolysin Proteins/biosynthesis,metabolism Hemolysis/genetics,physiology Lactones/analysis,chemistry Molecular Sequence Data Mutation Peptides, Cyclic Sequence Alignment Sequence Analysis, DNA Sequence Homology, Amino Acid Serine/metabolism Staphylococcus/genetics,metabolism
Chemicals
AgrD protein, Staphylococcus Bacterial Proteins DNA, Bacterial Hemolysin Proteins Lactones Peptides, Cyclic Serine
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Ji Guangyong
Departments of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA. gji@usuhs.mil
Pei Wuhong
Zhang Linsheng
Qiu Rongde
Lin Jianqun
Benito Yvonne
Lina Gerard
Novick Richard P
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.187.9.3139-3150.2005
Email
gji@usuhs.mil
Published
2005-05-00
Pages
3139-50
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC1082806
Subset
IM
Grants
NIAID NIH HHS · R01AI46445 · United States
NIAID NIH HHS · R01AI30138 · United States
NIAID NIH HHS · R01 AI042783 · United States
NIAID NIH HHS · R01 AI046445 · United States
NIAID NIH HHS · R01AI42783 · United States
NIAID NIH HHS · R01 AI030138 · United States
Databases
GENBANK
AF346723, AY557375, AY860843
External Links
PubMed source DOI: 10.1128/JB.187.9.3139-3150.2005 PMC Full Text View journal details
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