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PMID: 15831702 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

CD144 (VE-cadherin) is transiently expressed by fetal liver hematopoietic stem cells.

Blood ·Vol. 106 ·No. 3 ·2005-08-01 ·Pages 903-5

Kim I, Yilmaz OH, Morrison SJ

Abstract

Hematopoietic stem cells (HSCs) and endothelial progenitors arise from a common embryonic precursor. However, these populations diverge prior to the onset of definitive hematopoiesis, as HSCs become CD45+ and are thought to lose the expression of endothelial markers. After the onset of definitive hematopoiesis, CD144 (vascular endothelial [VE]-cadherin) has been considered a specific marker of endothelial cells. In contrast, we found that virtually all HSC activity from embryonic day 13.5 (E13.5) fetal liver was CD144+. CD144 expression declined on E16.5 fetal liver HSCs and was absent from adult bone marrow HSCs. This identified a new marker that is differentially expressed between fetal and adult HSCs, and enhanced the purification of HSCs from the E13.5 fetal liver. These results emphasize the close developmental relationship between hematopoietic and endothelial cells, while indicating that CD144 is not a specific marker of endothelial cells during fetal development.

MeSH Terms
Animals Antigens, CD Biomarkers/analysis Cadherins/analysis,genetics Endothelial Cells/chemistry Fetus/cytology Gene Expression Regulation, Developmental Hematopoietic Stem Cells/chemistry,metabolism Liver/cytology,embryology Mice Mice, Inbred C57BL
Chemicals
Antigens, CD Biomarkers Cadherins cadherin 5
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kim Injune
Howard Hughes Medical Institute, University of Michigan, 1500 E Medical Center Dr, 3215 CCGC, Ann Arbor, MI, 48109-0934, USA.
Yilmaz Omer H
Morrison Sean J
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Article Info
Journal
Blood
Abbr.
Blood
ISSN
0006-4971
DOI
10.1182/blood-2004-12-4960
Published
2005-08-01
Epub
2005-00-14
Pages
903-5
Language
English
Region
United States
NLM ID
7603509
PMCID
PMC1895147
Subset
IM
Grants
NIAMS NIH HHS · AR20 557 · United States
NCI NIH HHS · CA46 592 · United States
NIAMS NIH HHS · P30 AR48 310 · United States
NICHD NIH HHS · R21 HD40 760-02 · United States
External Links
PubMed source DOI: 10.1182/blood-2004-12-4960 PMC Full Text View journal details
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