Isolation of monochromosomal hybrids following fusion of human diploid fibroblast-derived microcells with mouse A9 cells.

Cytogenetics and cell genetics ·Vol. 60 ·No. 1 ·1992-00-00 ·Pages 79-80

Ning Y, Lovell M, Taylor L, Pereira-Smith OM

Abstract

Rodent x human hybrids containing single human chromosomes are valuable tools in the study of human genetics. Using a retrovirus carrying the neomycin gene, we generated a neomycin-resistant (neor) pool of human diploid fibroblasts. Following fusion of microcells prepared from this pool to mouse A9 cells, we obtained four neor hybrids containing a single human chromosome 2, 3, 5, or 15.

MeSH Terms

Animals Cell Fusion/genetics Cell Line Chromosomes, Human Chromosomes, Human, Pair 15 Chromosomes, Human, Pair 2 Chromosomes, Human, Pair 3 Chromosomes, Human, Pair 5 Drug Resistance/genetics Humans Hybrid Cells Mice Moloney murine leukemia virus/genetics Neomycin/pharmacology

Chemicals

Neomycin

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Ning Y

Roy M. and Phyllis Gough Huffington Center on Aging, Baylor College of Medicine, Houston, TX 77030.

Lovell M

Taylor L

Pereira-Smith O M

Article Info

Journal

Cytogenetics and cell genetics

Abbr.

Cytogenet Cell Genet

ISSN

0301-0171

DOI

10.1159/000133300

Published

1992-00-00

Pages

79-80

Language

English

Region

Switzerland

NLM ID

0367735

Subset

External Links

PubMed source DOI: 10.1159/000133300

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Isolation of monochromosomal hybrids following fusion of human diploid fibroblast-derived microcells with mouse A9 cells.

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