Abstract
The GTP/GDP nucleotide switch in Ras superfamily GTPases generally involves differential affinity toward downstream effectors, with the GTP-bound state having a higher affinity for effector than the GDP-bound state. We have developed a quantitative model of allosteric regulation of the Wiskott-Aldrich syndrome protein (WASP) by the Rho GTPase Cdc42 to better understand how GTPase binding is coupled to effector activation. The model accurately predicts WASP affinity for Cdc42, activity toward Arp2/3 complex, and activation by Cdc42 as functions of a two-state allosteric equilibrium in WASP. The ratio of GTPase affinities for the inactive and active states of WASP is appreciably larger for Cdc42-GTP than for Cdc42-GDP. The greater ability to distinguish between the two states of WASP makes Cdc42-GTP a full WASP agonist, whereas Cdc42-GDP is only a partial agonist. Thus, the nucleotide switch controls not only the affinity of Cdc42 for its effector but also the efficiency of coupling between the Cdc42-binding and allosteric equilibria in WASP. This effect can ensure high fidelity and specificity in Cdc42 signaling in crowded membrane environments.
MeSH Terms
Actin-Related Protein 2
Actin-Related Protein 3
Allosteric Regulation
Cytoskeletal Proteins/metabolism
Humans
Models, Molecular
Molecular Sequence Data
Protein Structure, Tertiary
Proteins/chemistry,genetics,metabolism
Wiskott-Aldrich Syndrome Protein
cdc42 GTP-Binding Protein/chemistry,genetics,metabolism
Chemicals
ACTR2 protein, human
ACTR3 protein, human
Actin-Related Protein 2
Actin-Related Protein 3
Cytoskeletal Proteins
Proteins
WAS protein, human
Wiskott-Aldrich Syndrome Protein
cdc42 GTP-Binding Protein
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Leung Daisy W
Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-8816, USA.
Rosen Michael K
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