Home LiteratureArticle Details
PMID: 15805469 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A novel cell-to-cell trafficking assay indicates that the KNOX homeodomain is necessary and sufficient for intercellular protein and mRNA trafficking.

Genes & development ·Vol. 19 ·No. 7 ·2005-04-01 ·Pages 788-93

Kim JY, Rim Y, Wang J, Jackson D

Abstract

Cell-to-cell trafficking of regulatory proteins is a novel mechanism for communication during cell fate specification in plants. Although several developmental proteins traffic cell-to-cell, no signals that are both necessary and sufficient for this function in developmental proteins have been described. We developed a novel trafficking assay using trichome rescue in Arabidopsis. Fusion to KNOTTED1 (KN1) conferred gain-of-trafficking function to the cell-autonomous GLABROUS1 (GL1) protein. We show that the KNOX homeodomain (HD) is necessary and sufficient for intercellular trafficking, identifying a novel function for the HD as the minimal sequence required for trafficking of KN1 and its associated mRNA.

MeSH Terms
Arabidopsis/metabolism Homeodomain Proteins/metabolism Plant Proteins/metabolism Protein Structure, Tertiary Protein Transport/physiology RNA, Messenger/metabolism Signal Transduction/physiology
Chemicals
Homeodomain Proteins Kn1 protein, plant Plant Proteins RNA, Messenger
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kim Jae-Yean
Division of Applied Life Science (BK21 program), Environmental Biotechnology National Core Research Center, PMBBRC, Gyeongsang National University, Jinju 660-701, Korea. kimjy@nongae.gsnu.ac.kr
Rim Yeonggil
Wang Jing
Jackson David
References (34)
34 references, click to expand
  1. Developmental changes due to long-distance movement of a homeobox fusion transcript in tomato.
    Science. 2001 Jul 13;293(5528):287-9 PMID: 11452121
  2. Subcellular localization determines the availability of non-targeted proteins to plasmodesmatal transport.
    Curr Biol. 2000 Sep 7;10(17):1032-40 PMID: 10996070
  3. The fasciated ear2 gene encodes a leucine-rich repeat receptor-like protein that regulates shoot meristem proliferation in maize.
    Genes Dev. 2001 Oct 15;15(20):2755-66 PMID: 11641280
  4. The Arabidopsis BELL1 and KNOX TALE homeodomain proteins interact through a domain conserved between plants and animals.
    Plant Cell. 2001 Nov;13(11):2455-70 PMID: 11701881
  5. Intercellular trafficking of a KNOTTED1 green fluorescent protein fusion in the leaf and shoot meristem of Arabidopsis.
    Proc Natl Acad Sci U S A. 2002 Mar 19;99(6):4103-8 PMID: 11891300
  6. The homeobox gene BREVIPEDICELLUS is a key regulator of inflorescence architecture in Arabidopsis.
    Proc Natl Acad Sci U S A. 2002 Apr 2;99(7):4730-5 PMID: 11917137
  7. ASYMMETRIC LEAVES1 reveals knox gene redundancy in Arabidopsis.
    Development. 2002 Apr;129(8):1957-65 PMID: 11934861
  8. Role of a positive regulator of root hair development, CAPRICE, in Arabidopsis root epidermal cell differentiation.
    Development. 2002 Dec;129(23):5409-19 PMID: 12403712
  9. Selective trafficking of non-cell-autonomous proteins mediated by NtNCAPP1.
    Science. 2003 Jan 17;299(5605):392-6 PMID: 12532017
  10. Modes of intercellular transcription factor movement in the Arabidopsis apex.
    Development. 2003 Aug;130(16):3735-45 PMID: 12835390
  11. Phyllotactic pattern and stem cell fate are determined by the Arabidopsis homeobox gene BELLRINGER.
    Development. 2003 Sep;130(17):3941-50 PMID: 12874117
  12. Developmental regulation and significance of KNOX protein trafficking in Arabidopsis.
    Development. 2003 Sep;130(18):4351-62 PMID: 12900451
  13. A broad competence to respond to SHORT ROOT revealed by tissue-specific ectopic expression.
    Development. 2004 Jun;131(12):2817-26 PMID: 15142972
  14. Mechanisms regulating SHORT-ROOT intercellular movement.
    Curr Biol. 2004 Oct 26;14(20):1847-51 PMID: 15498493
  15. The P30 movement protein of tobacco mosaic virus is a single-strand nucleic acid binding protein.
    Cell. 1990 Feb 23;60(4):637-47 PMID: 2302736
  16. Transgene expression variability (position effect) of CAT and GUS reporter genes driven by linked divergent T-DNA promoters.
    Plant Mol Biol. 1991 Jul;17(1):49-60 PMID: 1907871
  17. A myb gene required for leaf trichome differentiation in Arabidopsis is expressed in stipules.
    Cell. 1991 Nov 1;67(3):483-93 PMID: 1934056
  18. Direct functional assay for tobacco mosaic virus cell-to-cell movement protein and identification of a domain involved in increasing plasmodesmal permeability.
    Proc Natl Acad Sci U S A. 1994 Feb 15;91(4):1433-7 PMID: 8108427
  19. A knotted1-like homeobox gene in Arabidopsis is expressed in the vegetative meristem and dramatically alters leaf morphology when overexpressed in transgenic plants.
    Plant Cell. 1994 Dec;6(12):1859-76 PMID: 7866029
  20. Sequence analysis and expression patterns divide the maize knotted1-like homeobox genes into two classes.
    Plant Cell. 1994 Dec;6(12):1877-87 PMID: 7866030
  21. Selective trafficking of KNOTTED1 homeodomain protein and its mRNA through plasmodesmata.
    Science. 1995 Dec 22;270(5244):1980-3 PMID: 8533088
  22. A member of the KNOTTED class of homeodomain proteins encoded by the STM gene of Arabidopsis.
    Nature. 1996 Jan 4;379(6560):66-9 PMID: 8538741
  23. RNA recognition and translational regulation by a homeodomain protein.
    Nature. 1996 Feb 22;379(6567):694-9 PMID: 8602214
  24. Tobacco mosaic virus movement protein-mediated protein transport between trichome cells.
    Plant Cell. 1995 Dec;7(12):2069-79 PMID: 8718620
  25. Non-cell-autonomous function of the Antirrhinum floral homeotic proteins DEFICIENS and GLOBOSA is exerted by their polar cell-to-cell trafficking.
    Development. 1996 Nov;122(11):3433-41 PMID: 8951059
  26. Cell-to-cell movement of potato spindle tuber viroid.
    Plant J. 1997 Oct;12(4):931-6 PMID: 9375403
  27. Control of GL2 expression in Arabidopsis leaves and trichomes.
    Development. 1998 Apr;125(7):1161-71 PMID: 9477315
  28. Role of WUSCHEL in regulating stem cell fate in the Arabidopsis shoot meristem.
    Cell. 1998 Dec 11;95(6):805-15 PMID: 9865698
  29. Plant paralog to viral movement protein that potentiates transport of mRNA into the phloem.
    Science. 1999 Jan 1;283(5398):94-8 PMID: 9872750
  30. Simple, but not branched, plasmodesmata allow the nonspecific trafficking of proteins in developing tobacco leaves.
    Cell. 1999 Jun 11;97(6):743-54 PMID: 10380926
  31. Movement protein of tobacco mosaic virus modifies plasmodesmatal size exclusion limit.
    Science. 1989 Oct 20;246(4928):377-9 PMID: 16552920
  32. Knots in the family tree: evolutionary relationships and functions of knox homeobox genes.
    Plant Mol Biol. 2000 Jan;42(1):151-66 PMID: 10688134
  33. Peptide antagonists of the plasmodesmal macromolecular trafficking pathway.
    EMBO J. 2000 Jun 15;19(12):2856-68 PMID: 10856231
  34. Intercellular movement of the putative transcription factor SHR in root patterning.
    Nature. 2001 Sep 20;413(6853):307-11 PMID: 11565032
Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.332805
Email
kimjy@nongae.gsnu.ac.kr
Published
2005-04-01
Pages
788-93
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC1074316
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.332805 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2