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PMID: 15800998 Published · ppublish English Journal Article

Effects of psychological stress on small intestinal motility and bacteria and mucosa in mice.

World journal of gastroenterology ·Vol. 11 ·No. 13 ·2005-04-07 ·Pages 2016-21

Wang SX, Wu WC

Abstract

To investigate the effects of psychological stress on small intestinal motility and bacteria and mucosa in mice, and to explore the relationship between small intestinal dysfunction and small intestinal motility and bacteria and mucosa under psychological stress. Sixty mice were randomly divided into psychological stress group and control group. Each group were subdivided into small intestinal motility group (n = 10), bacteria group (n = 10), and D-xylose administered to stomach group (n = 10). An animal model with psychological stress was established housing the mice with a hungry cat in separate layers of a two-layer cage. A semi-solid colored marker (carbon-ink) was used for monitoring small intestinal transit. The proximal small intestine was harvested under sterile condition and processed for quantitation for aerobes (Escherichia coli) and anaerobes (Lactobacilli). The quantitation of bacteria was expressed as log10(colony forming units/g). D-xylose levels in plasma were measured for estimating the damage of small intestinal mucosa. Small intestinal transit was inhibited (39.80+/-9.50% vs 58.79+/-11.47%, P<0.01) in mice after psychological stress, compared with the controls. Psychological stress resulted in quantitative alterations in the aerobes (E. coli). There was an increase in the number of E. coli in the proximal small intestinal flora (1.78+/-0.30 log10 (CFU/g) vs 1.37+/-0.21 log10 (CFU/g), P<0.01), and there was decrease in relative proportion of Lactobacilli and E. coli of stressed mice (0.53+/-0.63 vs 1.14+/-1.07, P<0.05), while there was no significant difference in the anaerobes (Lactobacilli) between the two groups (2.31+/-0.70 log10 (CFU/g) vs 2.44+/-0.37 log10 (CFU/g), P>0.05). D-xylose concentrations in plasma in psychological stress mice were significantly higher than those in the control group (2.90+/-0.89 mmol/L vs 0.97+/-0.33 mmol/L, P<0.01). Small intestinal dysfunction under psychological stress may be related to the small intestinal motility disorder and dysbacteriosis and the damage of mucosa probably caused by psychological stress.

MeSH Terms
Animals Bacteria, Aerobic/growth & development Escherichia coli/genetics Gastrointestinal Motility/physiology Intestinal Mucosa/microbiology,physiology Intestine, Small/microbiology,physiology Lactobacillus/growth & development Male Mice Stress, Psychological/physiopathology Xylose/blood
Chemicals
Xylose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wang Shao-Xuan
Department of Gastroenterology, Yijishan Hospital, Wuhu 241001, Anhui Province, China. wang51607@163.com
Wu Wan-Chun
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Article Info
Journal
World journal of gastroenterology
Abbr.
World J Gastroenterol
ISSN
1007-9327
DOI
10.3748/wjg.v11.i13.2016
Email
wang51607@163.com
Published
2005-04-07
Pages
2016-21
Language
English
Region
United States
NLM ID
100883448
PMCID
PMC4305729
Subset
IM
External Links
PubMed source DOI: 10.3748/wjg.v11.i13.2016 PMC Full Text View journal details
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