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PMID: 15791208 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Role of curvature and phase transition in lipid sorting and fission of membrane tubules.

The EMBO journal ·Vol. 24 ·No. 8 ·2005-04-20 ·Pages 1537-45

Roux A, Cuvelier D, Nassoy P, Prost J, Bassereau P, Goud B

Abstract

We have recently developed a minimal system for generating long tubular nanostructures that resemble tubes observed in vivo with biological membranes. Here, we studied membrane tube pulling in ternary mixtures of sphingomyelin, phosphatidylcholine and cholesterol. Two salient results emerged: the lipid composition is significantly different in the tubes and in the vesicles; tube fission is observed when phase separation is generated in the tubes. This shows that lipid sorting may depend critically on both membrane curvature and phase separation. Phase separation also appears to be important for membrane fission in tubes pulled out of giant liposomes or purified Golgi membranes.

MeSH Terms
Brain Chemistry Cell Membrane/chemistry,metabolism Cholesterol/chemistry,metabolism Elasticity Golgi Apparatus/chemistry Lipid Bilayers/chemistry Phosphatidylcholines/chemistry,metabolism Photochemistry Sphingomyelins/chemistry,metabolism Stress, Mechanical
Chemicals
Lipid Bilayers Phosphatidylcholines Sphingomyelins Cholesterol
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Roux Aurélien
UMR 144 CNRS/Institut Curie, Paris, France.
Cuvelier Damien
Nassoy Pierre
Prost Jacques
Bassereau Patricia
Goud Bruno
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1038/sj.emboj.7600631
Published
2005-04-20
Epub
2005-00-24
Pages
1537-45
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1142567
Subset
IM
External Links
PubMed source DOI: 10.1038/sj.emboj.7600631 PMC Full Text View journal details
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