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PMID: 15790861 Published · ppublish English Comparative Study Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The role of hyaluronan synthase 3 in ventilator-induced lung injury.

American journal of respiratory and critical care medicine ·Vol. 172 ·No. 1 ·2005-07-01 ·Pages 92-8

Bai KJ, Spicer AP, Mascarenhas MM, Yu L, Ochoa CD, Garg HG, Quinn DA

Abstract

We recently found that low-molecular-weight hyaluronan was induced by cyclic stretch in lung fibroblasts and accumulated in lungs from animals with ventilator-induced lung injury. The low-molecular-weight hyaluronan produced by stretch increased interleukin-8 production in epithelial cells, and was accompanied by an upregulation of hyaluronan synthase-3 mRNA. We hypothesized that low-molecular-weight hyaluronan induced by high VT was dependent on hyaluronan synthase 3, and was associated with ventilator-induced lung injury. Effects of high VT ventilation in C57BL/6 wild-type and hyaluronan synthase-3 knockout mice were compared. Significantly increased neutrophil infiltration, macrophage inflammatory protein-2 production, and lung microvascular leak were found in wild-type animals ventilated with high VT. These reactions were significantly reduced in hyaluronan synthase-3 knockout mice, except the capillary leak. Wild-type mice ventilated with high VT were found to have increased low-molecular-weight hyaluronan in lung tissues and concomitant increased expression of hyaluronan synthase-3 mRNA, neither of which was found in hyaluronan synthase-3 knockout mice. We conclude that high VT induced low-molecular-weight hyaluronan production is dependent on de novo synthesis through hyaluronan synthase 3, and plays a role in the inflammatory response of ventilator-induced lung injury.

MeSH Terms
Algorithms Animals Chemokine CXCL2 Disease Models, Animal Female Glucuronosyltransferase/biosynthesis,genetics Hyaluronan Synthases Interleukin-8/metabolism Lung/enzymology Lung Diseases/enzymology,etiology Lung Injury Male Mice Mice, Inbred C57BL Mice, Knockout Monokines/metabolism Respiration, Artificial/adverse effects Tidal Volume
Chemicals
Chemokine CXCL2 Interleukin-8 Monokines Glucuronosyltransferase Has3 protein, mouse Hyaluronan Synthases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Bai Kuan-Jen
Pulmonary and Critical Care Unit, Massachusetts General Hospital, 55 Fruit Street, Bulfinch-148, Boston, MA 02114, USA.
Spicer Andrew P
Mascarenhas Marcella M
Yu Lunyin
Ochoa Cristhiaan D
Garg Hari G
Quinn Deborah A
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41 references, click to expand
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Article Info
Journal
American journal of respiratory and critical care medicine
Abbr.
Am J Respir Crit Care Med
ISSN
1073-449X
DOI
10.1164/rccm.200405-652OC
Published
2005-07-01
Epub
2005-00-24
Pages
92-8
Language
English
Region
United States
NLM ID
9421642
PMCID
PMC2718450
Subset
IM
Grants
NHLBI NIH HHS · 2T32 HL 07874 · United States
NHLBI NIH HHS · HL 03920 · United States
External Links
PubMed source DOI: 10.1164/rccm.200405-652OC PMC Full Text View journal details
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