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PMID: 15781470 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Membrane topology of the STT3 subunit of the oligosaccharyl transferase complex.

The Journal of biological chemistry ·Vol. 280 ·No. 21 ·2005-05-27 ·Pages 20261-7

Kim H, von Heijne G, Nilsson I

Abstract

The highly conserved membrane protein STT3 is part of the oligosaccharyl transferase complex in the endoplasmic reticulum of eukaryotic cells. Various experimental observations strongly suggest that STT3 contains the active site of the complex. Here, we report a detailed topology study of STT3 from two different organisms, Saccharomyces cerevisiae and mouse, using in vivo and in vitro topology mapping assays. Our results suggest that STT3 has 11 transmembrane helices and an overall N(cyt)-C(lum) orientation.

MeSH Terms
Animals Binding Sites Blotting, Western Cell Membrane/enzymology Cloning, Molecular Electrophoresis, Polyacrylamide Gel Glycosylation Hexosyltransferases/chemistry,genetics Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase/metabolism Membrane Proteins/chemistry,genetics Mice Models, Molecular Mutagenesis, Site-Directed Peptide Fragments/chemistry,metabolism Protein Structure, Secondary Recombinant Fusion Proteins Recombinant Proteins Saccharomyces cerevisiae/enzymology Saccharomyces cerevisiae Proteins/chemistry,genetics beta-Fructofuranosidase/chemistry,genetics
Chemicals
Membrane Proteins Peptide Fragments Recombinant Fusion Proteins Recombinant Proteins STT3-A protein, mouse Saccharomyces cerevisiae Proteins Hexosyltransferases STT3 protein, S cerevisiae dolichyl-diphosphooligosaccharide - protein glycotransferase beta-Fructofuranosidase Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kim Hyun
Department of Biochemistry and Biophysics, Stockholm University, SE-10691 Stockholm, Sweden.
von Heijne Gunnar
Nilsson IngMarie
Article Info
Journal
The Journal of biological chemistry
Abbr.
J Biol Chem
ISSN
0021-9258
DOI
10.1074/jbc.M412213200
Published
2005-05-27
Epub
2005-00-21
Pages
20261-7
Language
English
Region
United States
NLM ID
2985121R
Subset
IM
External Links
PubMed source DOI: 10.1074/jbc.M412213200 View journal details
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