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PMID: 1577691 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Conserved gene arrangement in the origin region of the Streptomyces coelicolor chromosome.

Journal of bacteriology ·Vol. 174 ·No. 10 ·1992-05-00 ·Pages 3220-6

Calcutt MJ, Schmidt FJ

Abstract

A 23-kb fragment of the Streptomyces coelicolor chromosome spanning the dnaA region has been isolated as a cosmid clone. Nucleotide sequence analysis of a 5-kb portion shows that the genes for the RNase P protein (rnpA), ribosomal protein L34 (rpmH), the replication initiator protein (dnaA), and the beta subunit of DNA polymerase III (dnaN) are present in the highly conserved gene arrangement found in all eubacterial genomes studied so far. The dnaA-dnaN intergenic region is approximately 1 kb and contains a cluster of at least 12 DnaA boxes with a consensus sequence of TTGTCCACA matching the consensus DnaA box in the phylogenetically related Micrococcus luteus. Two DnaA boxes precede the dnaA sequence. We propose that the chromosomal origin (oriC) of S. coelicolor lies between dnaA and dnaN. In related work, J. Zakrzewska-Czerwinska and H. Schrempf (J. Bacteriol. 174:2688-2693, 1992) have identified the homologous sequence from the closely-related Streptomyces lividans as capable of self-replication.

Related Genes

dnaA dnaN rnpA rpmH

MeSH Terms

Amino Acid Sequence Animals Bacterial Proteins/genetics Base Sequence Chromosome Mapping Cloning, Molecular Consensus Sequence Cosmids DNA Polymerase III/genetics DNA Replication/genetics DNA-Binding Proteins/genetics Dogs Endoribonucleases/genetics Genes, Bacterial/genetics Molecular Sequence Data Phylogeny RNA, Catalytic/genetics Regulatory Sequences, Nucleic Acid Ribonuclease P Ribosomal Proteins/genetics Sequence Homology, Nucleic Acid Streptomyces/genetics

Chemicals

Bacterial Proteins DNA-Binding Proteins DnaA protein, Bacteria RNA, Catalytic Ribosomal Proteins ribosomal protein L34 DNA Polymerase III Endoribonucleases Ribonuclease P

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Calcutt M J

Department of Biochemistry, University of Missouri-Columbia 65212.

Schmidt F J

References (19)

19 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.174.10.3220-3226.1992

Published

1992-05-00

Pages

3220-6

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC205989

Subset

Databases

GENBANK

M79367, M79368, M79369, M79370, M79371, M79372, M82836, M87491, M87492, M87836

External Links

PubMed source DOI: 10.1128/jb.174.10.3220-3226.1992 PMC Full Text View journal details

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Conserved gene arrangement in the origin region of the Streptomyces coelicolor chromosome.

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