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PMID: 15711810 Published · ppublish English Journal Article

Global analysis of fluorescence fluctuation data.

European biophysics journal : EBJ ·Vol. 34 ·No. 4 ·2005-06-00 ·Pages 323-34

Skakun VV, Hink MA, Digris AV, Engel R, Novikov EG, Apanasovich VV, Visser AJ

Abstract

Over the last decade the number of applications of fluorescence correlation spectroscopy (FCS) has grown rapidly. Here we describe the development and application of a software package, FCS Data Processor, to analyse the acquired correlation curves. The algorithms combine strong analytical power with flexibility in use. It is possible to generate initial guesses, link and constrain fit parameters to improve the accuracy and speed of analysis. A global analysis approach, which is most effective in analysing autocorrelation curves determined from fluorescence fluctuations of complex biophysical systems, can also be implemented. The software contains a library of frequently used models that can be easily extended to include user-defined models. The use of the software is illustrated by analysis of different experimental fluorescence fluctuation data sets obtained with Rhodamine Green in aqueous solution and enhanced green fluorescent protein in vitro and in vivo.

MeSH Terms
Algorithms Animals Biophysics/methods Calibration Dictyostelium/metabolism Diffusion Fluorescent Dyes/pharmacology Green Fluorescent Proteins/metabolism Hydrogen-Ion Concentration Image Interpretation, Computer-Assisted/methods Microscopy, Fluorescence/methods Models, Statistical Models, Theoretical Phosphates/chemistry Rhodamines/pharmacology Software Statistics as Topic/methods Time Factors
Chemicals
Fluorescent Dyes Phosphates Rhodamines Green Fluorescent Proteins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Skakun Victor V
Department of Systems Analysis, Belarusian State University, Minsk 220050, Belarus.
Hink Mark A
Digris Anatoli V
Engel Ruchira
Novikov Eugene G
Apanasovich Vladimir V
Visser Antonie J W G
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Article Info
Journal
European biophysics journal : EBJ
Abbr.
Eur Biophys J
ISSN
0175-7571
DOI
10.1007/s00249-004-0453-9
Published
2005-06-00
Epub
2005-00-12
Pages
323-34
Language
English
Region
Germany
NLM ID
8409413
Subset
IM
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1007/s00249-004-0453-9 View journal details
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