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PMID: 15695360 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Mitochondrial translation: elongation factor tu is essential in fission yeast and depends on an exchange factor conserved in humans but not in budding yeast.

Genetics ·Vol. 169 ·No. 4 ·2005-04-00 ·Pages 1891-901

Chiron S, Suleau A, Bonnefoy N

Abstract

The translation elongation factor EF-Tu is a GTPase that delivers amino-acylated tRNAs to the ribosome during the elongation step of translation. EF-Tu/GDP is recycled by the guanine nucleotide exchange factor EF-Ts. Whereas EF-Ts is lacking in S. cerevisiae, both translation factors are found in S. pombe and H. sapiens mitochondria, consistent with the known similarity between fission yeast and human cell mitochondrial physiology. We constructed yeast mutants lacking these elongation factors. We show that mitochondrial translation is vital for S. pombe, as it is for human cells. In a genetic background allowing the loss of mitochondrial functions, a block in mitochondrial translation in S. pombe leads to a major depletion of mtDNA. The relationships between EF-Ts and EF-Tu from both yeasts and humans were investigated through functional complementation and coexpression experiments and by a search for suppressors of the absence of the S. pombe EF-Ts. We find that S. cerevisiae EF-Tu is functionally equivalent to the S. pombe EF-Tu/EF-Ts couple. Point mutations in the S. pombe EF-Tu can render it independent of its exchange factor, thereby mimicking the situation in S. cerevisiae.

MeSH Terms
Amino Acid Sequence Base Sequence DNA, Mitochondrial/genetics Gene Deletion Genetic Complementation Test Genetic Techniques Glucose/metabolism Humans Mitochondria/genetics,metabolism,pathology,physiology Models, Molecular Molecular Sequence Data Mutation Oxygen Consumption Peptide Elongation Factor Tu/physiology Phenotype Point Mutation Protein Binding Protein Biosynthesis Saccharomyces cerevisiae/genetics,physiology Schizosaccharomyces/genetics,physiology Sequence Analysis, DNA Species Specificity Transcription, Genetic
Chemicals
DNA, Mitochondrial Peptide Elongation Factor Tu Glucose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Chiron Stéphane
Centre de Génétique Moléculaire du CNRS, Gif-sur-Yvette, France.
Suleau Audrey
Bonnefoy Nathalie
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
Published
2005-04-00
Epub
2005-00-03
Pages
1891-901
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1449603
Subset
IM
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