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PMID: 15687504 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Conserved patterns of protein interaction in multiple species.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 102 ·No. 6 ·2005-02-08 ·Pages 1974-9

Sharan R, Suthram S, Kelley RM, Kuhn T, McCuine S, Uetz P, Sittler T, Karp RM, Ideker T

Abstract

To elucidate cellular machinery on a global scale, we performed a multiple comparison of the recently available protein-protein interaction networks of Caenorhabditis elegans, Drosophila melanogaster, and Saccharomyces cerevisiae. This comparison integrated protein interaction and sequence information to reveal 71 network regions that were conserved across all three species and many exclusive to the metazoans. We used this conservation, and found statistically significant support for 4,645 previously undescribed protein functions and 2,609 previously undescribed protein interactions. We tested 60 interaction predictions for yeast by two-hybrid analysis, confirming approximately half of these. Significantly, many of the predicted functions and interactions would not have been identified from sequence similarity alone, demonstrating that network comparisons provide essential biological information beyond what is gleaned from the genome.

MeSH Terms
Amino Acid Sequence Animals Caenorhabditis elegans Proteins/genetics,metabolism Databases, Nucleic Acid Drosophila Proteins/genetics,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism Sequence Alignment Sequence Homology, Nucleic Acid Two-Hybrid System Techniques
Chemicals
Caenorhabditis elegans Proteins Drosophila Proteins Saccharomyces cerevisiae Proteins
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Sharan Roded
Computer Science Division, University of California, Berkeley, CA 94704, USA.
Suthram Silpa
Kelley Ryan M
Kuhn Tanja
McCuine Scott
Uetz Peter
Sittler Taylor
Karp Richard M
Ideker Trey
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0409522102
Published
2005-02-08
Epub
2005-00-01
Pages
1974-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC548573
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.0409522102 PMC Full Text View journal details
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