Home LiteratureArticle Details

PMID: 15683403 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Frequent genetic recombination in natural populations of the marine cyanobacterium Microcoleus chthonoplastes.

Environmental microbiology ·Vol. 7 ·No. 3 ·2005-03-00 ·Pages 434-42

Lodders N, Stackebrandt E, Nübel U

Abstract

A culture-independent method for multilocus sequence typing of Microcoleus chthonoplastes was developed based on mechanical separation of individual cyanobacterial filaments from natural microbial mat populations through micromanipulation, subsequent polymerase chain reaction (PCR) amplification and sequence analysis of three genetic loci (kaiC, petB/D, rDNA-ITS). Among 81 individuals sampled from intertidal sand flats of the North Sea and Baltic Sea, we found 8-14 different sequences (alleles) per genetic locus, resulting in 36 distinct genotypes with unique allele profiles. Non-congruent phylogenetic gene trees for the three loci analysed and split decomposition analysis indicated the occurrence of horizontal genetic exchange. The index of association determined for the entire population was 0.096, indicating that recombination occurs frequently enough to cause almost random association (linkage equilibrium) among alleles. Analysing individuals from three different locations in the North Sea and Baltic Sea, we did not find evidence for geographic subdivisions between populations.

MeSH Terms

Alleles Bacterial Typing Techniques Baltic States Cyanobacteria/genetics,isolation & purification Genetics, Population Molecular Sequence Data North Sea Phylogeny Recombination, Genetic Seawater/microbiology

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Lodders Nicole

Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, Germany.

Stackebrandt Erko

Nübel Ulrich

Article Info

Journal

Environmental microbiology

Abbr.

Environ Microbiol

ISSN

1462-2912

DOI

10.1111/j.1462-2920.2005.00730.x

Published

2005-03-00

Pages

434-42

Language

English

Region

England

NLM ID

100883692

Subset

Databases

GENBANK

AJ809630, AJ809631, AJ809632, AJ809633, AJ809634, AJ809635, AJ809636, AJ809637, AJ809638, AJ809639, AJ809640, AJ809641, AJ809642, AJ809643, AJ809644, AJ809645, AJ809646, AJ809647, AJ809648, AJ809649, AJ809650, AJ809651, AJ809652, AJ809653, AJ809654, AJ809655, AJ809656, AJ809657, AJ809658, AJ809659, AJ809660, AJ809661, AJ809662, AJ809663, AJ809664, AJ809665, AJ809666, AJ809667, AJ809668, AJ809669, AJ809670, AJ809671, AJ809672, AJ809673, AJ809674, AJ809675, AJ809676, AJ809677, AJ809678, AJ809679, AJ809680, AJ809681, AJ809682, AJ809683, AJ809684, AJ809685, AJ809686, AJ809687, AJ809688, AJ809689, AJ809690, AJ809691, AJ809692, AJ809693, AJ809694, AJ809695, AJ809696, AJ809697, AJ809698, AJ809699, AJ809700, AJ809701, AJ809702, AJ809703, AJ809704, AJ809705, AJ809706, AJ809707, AJ809708, AJ809709, AJ809710, AJ809711, AJ809712, AJ809713, AJ809714, AJ809715, AJ809716, AJ809717, AJ809718, AJ809719, AJ809720, AJ809721, AJ809722, AJ809723, AJ809724, AJ809725, AJ809726, AJ809727, AJ809728, AJ809729, AJ809730, AJ809731, AJ809732, AJ809733, AJ809734, AJ809735, AJ809736, AJ809737, AJ809738, AJ809739, AJ809740, AJ809741, AJ809742, AJ809743, AJ809744, AJ809745, AJ809746, AJ809747, AJ809748, AJ809749, AJ809750, AJ809751, AJ809752, AJ809753, AJ809754, AJ809755, AJ809756, AJ809757, AJ809758, AJ809759, AJ809760, AJ809761, AJ809762, AJ809763, AJ809764, AJ809765, AJ809766, AJ809767, AJ809768, AJ809769, AJ809770, AJ809771, AJ809772, AJ809773, AJ809774, AJ809775, AJ809776, AJ809777, AJ809778, AJ809779, AJ809780, AJ809781, AJ809782, AJ809783, AJ809784, AJ809785, AJ809786, AJ809787, AJ809788, AJ809789, AJ809790, AJ809791, AJ809792, AJ809793, AJ809794, AJ809795, AJ809796, AJ809797, AJ809798, AJ809799, AJ809800, AJ809801, AJ809802, AJ809803, AJ809804, AJ809805, AJ809806, AJ809807, AJ809808, AJ809809, AJ809810, AJ809811, AJ809812, AJ809813, AJ809814, AJ809815, AJ809816, AJ809817, AJ809818, AJ809819, AJ809820, AJ809821, AJ809822, AJ809823, AJ809824, AJ809825, AJ809826, AJ809827, AJ809828, AJ809829, AJ809830, AJ809831, AJ809832, AJ809833, AJ809834, AJ809835, AJ809836, AJ809837, AJ809838, AJ809839, AJ809840, AJ809841, AJ809842, AJ809843, AJ809844, AJ809845, AJ809846, AJ809847, AJ809848, AJ809849, AJ809850, AJ809851, AJ809852, AJ809853, AJ809854, AJ809855, AJ809856, AJ809857, AJ809858, AJ809859, AJ809860, AJ809861, AJ809862, AJ809863, AJ809864, AJ809865, AJ809866, AJ809867, AJ809868, AJ809869, AJ809870, AJ809871, AJ809872

External Links

PubMed source DOI: 10.1111/j.1462-2920.2005.00730.x View journal details

Analysis Services

Frequent genetic recombination in natural populations of the marine cyanobacterium Microcoleus chthonoplastes.

Contact

WeChat Official Account