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PMID: 15678100 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Staphylococcus aureus RNAIII and the endoribonuclease III coordinately regulate spa gene expression.

The EMBO journal ·Vol. 24 ·No. 4 ·2005-02-23 ·Pages 824-35

Huntzinger E, Boisset S, Saveanu C, Benito Y, Geissmann T, Namane A, Lina G, Etienne J, Ehresmann B, Ehresmann C, Jacquier A, Vandenesch F, Romby P

Abstract

Staphylococcus aureus RNAIII is one of the largest regulatory RNAs, which controls several virulence genes encoding exoproteins and cell-wall-associated proteins. One of the RNAIII effects is the repression of spa gene (coding for the surface protein A) expression. Here, we show that spa repression occurs not only at the transcriptional level but also by RNAIII-mediated inhibition of translation and degradation of the stable spa mRNA by the double-strand-specific endoribonuclease III (RNase III). The 3' end domain of RNAIII, partially complementary to the 5' part of spa mRNA, efficiently anneals to spa mRNA through an initial loop-loop interaction. Although this annealing is sufficient to inhibit in vitro the formation of the translation initiation complex, the coordinated action of RNase III is essential in vivo to degrade the mRNA and irreversibly arrest translation. Our results further suggest that RNase III is recruited for targeting the paired RNAs. These findings add further complexity to the expression of the S. aureus virulon.

MeSH Terms
Antigens, Bacterial/genetics,metabolism Bacterial Proteins/metabolism Base Sequence Binding Sites Gene Expression Regulation, Bacterial Molecular Sequence Data Nucleic Acid Conformation Protein Biosynthesis RNA Stability RNA, Antisense/chemistry,genetics,metabolism RNA, Bacterial/chemistry,genetics,metabolism RNA, Messenger/genetics,metabolism Ribonuclease III/metabolism Ribosomes/metabolism Staphylococcal Protein A/genetics,metabolism Staphylococcus aureus/enzymology,genetics Transcription, Genetic/genetics
Chemicals
Antigens, Bacterial Bacterial Proteins RNA, Antisense RNA, Bacterial RNA, Messenger RNAIII, Staphylococcus aureus Staphylococcal Protein A streptococcal protective antigen Ribonuclease III
Authors & Affiliations
13 authors, click to expand affiliations / ORCID
Huntzinger Eric
UPR 9002 du CNRS, Institut de Biologie Moléculaire et Cellulaire, Strasbourg Cedex, France.
Boisset Sandrine
Saveanu Cosmin
Benito Yvonne
Geissmann Thomas
Namane Abdelkader
Lina Gérard
Etienne Jerome
Ehresmann Bernard
Ehresmann Chantal
Jacquier Alain
Vandenesch François
Romby Pascale
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1038/sj.emboj.7600572
Published
2005-02-23
Epub
2005-00-27
Pages
824-35
Language
English
Region
England
NLM ID
8208664
PMCID
PMC549626
Subset
IM
External Links
PubMed source DOI: 10.1038/sj.emboj.7600572 PMC Full Text View journal details
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