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PMID: 15672866 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Inostamycin suppresses vascular endothelial growth factor-stimulated growth and migration of human umbilical vein endothelial cells.

Clinical & experimental metastasis ·Vol. 21 ·No. 5 ·2004-00-00 ·Pages 419-25

Baba Y, Kato Y, Mochimatsu I, Nagashima Y, Kurihara M, Kawano T, Taguchi T, Hata R, Tsukuda M

Abstract

Angiogenesis involves multiple steps including proliferation and migration of endothelial cells. In the present study, we determined the effect of inostamycin (an inhibitor of phosphatidylinositol synthesis) on vascular endothelial growth factor (VEGF)-induced proliferation and migration of human umbilical vein endothelial cells (HUVECs). Inostamycin significantly attenuated both VEGF-induced proliferation and migration of HUVECs. Inostamycin inhibited activation of mitogen-activated kinases (ERK and p38) and elevation of cyclin D1 induced by VEGF. These data suggest that inostamycin reduced both proliferation and migration of HUVECs by targeting ERK-cyclin D1 and p38, respectively.

MeSH Terms
Cell Movement/drug effects Cell Proliferation/drug effects Cells, Cultured Cyclin D1/pharmacology Endothelial Cells/drug effects Furans/pharmacology Humans Mitogen-Activated Protein Kinase 1/metabolism Mitogen-Activated Protein Kinase 3/metabolism Umbilical Veins/drug effects Vascular Endothelial Growth Factor A/metabolism,pharmacology p38 Mitogen-Activated Protein Kinases/metabolism
Chemicals
Furans Vascular Endothelial Growth Factor A inostamycin Cyclin D1 Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 p38 Mitogen-Activated Protein Kinases
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Baba Yuh
Department of Biology and Function in the Head and Neck, Yokohama City University Graduate School of Medicine, Yokohama, Japan.
Kato Yasumasa
Mochimatsu Izumi
Nagashima Yoji
Kurihara Miki
Kawano Toshiro
Taguchi Takahide
Hata Ryu-Ichiro
Tsukuda Mamoru
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Article Info
Journal
Clinical & experimental metastasis
Abbr.
Clin Exp Metastasis
ISSN
0262-0898
DOI
10.1007/s10585-005-0041-0
Published
2004-00-00
Pages
419-25
Language
English
Region
Netherlands
NLM ID
8409970
Subset
IM
External Links
PubMed source DOI: 10.1007/s10585-005-0041-0 View journal details
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