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PMID: 15655834 Published · ppublish English Journal Article

Effects of psychological stress on small intestinal motility and expression of cholecystokinin and vasoactive intestinal polypeptide in plasma and small intestine in mice.

World journal of gastroenterology ·Vol. 11 ·No. 5 ·2005-02-07 ·Pages 737-40

Cao SG, Wu WC, Han Z, Wang MY

Abstract

To investigate the effects of psychological stress on small intestinal motility and expression of cholecystokinin (CCK) and vasoactive intestinal polypeptide (VIP) in plasma and small intestine, and to explore the relationship between small intestinal motor disorders and gastrointestinal hormones under psychological stress. Thirty-six mice were randomly divided into psychological stress group and control group. A mouse model with psychological stress was established by housing the mice with a hungry cat in separate layers of a two-layer cage. A semi-solid colored marker (carbon-ink) was used for monitoring small intestinal transit. CCK and VIP levels in plasma and small intestine in mice were measured by radioimmunoassay (RIA). Small intestinal transit was inhibited (52.18+/-19.15% vs 70.19+/-17.79%, P<0.01) in mice after psychological stress, compared to the controls. Small intestinal CCK levels in psychological stress mice were significantly lower than those in the control group (0.75+/-0.53 microg/g vs 1.98+/-1.17 microg/g, P<0.01), whereas plasma CCK concentrations were not different between the groups. VIP levels in small intestine were significantly higher in psychological stress mice than those in the control group (8.45+/-1.09 microg/g vs 7.03+/-2.36 microg/g, P<0.01), while there was no significant difference in plasma VIP levels between the two groups. Psychological stress inhibits the small intestinal transit, probably by down-regulating CCK and up-regulating VIP expression in small intestine.

MeSH Terms
Animals Cats Cholecystokinin/blood Down-Regulation Fear/physiology Gastrointestinal Motility/physiology Intestine, Small/metabolism,physiopathology Male Mice Stress, Psychological/blood,physiopathology Up-Regulation Vasoactive Intestinal Peptide/blood
Chemicals
Vasoactive Intestinal Peptide Cholecystokinin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Cao Shu-Guang
Department of Gastroenterology, Yijishan Hospital, Wuhu 241001, Anhui Province, China.
Wu Wan-Chun
Han Zhen
Wang Meng-Ya
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Article Info
Journal
World journal of gastroenterology
Abbr.
World J Gastroenterol
ISSN
1007-9327
DOI
10.3748/wjg.v11.i5.737
Published
2005-02-07
Pages
737-40
Language
English
Region
United States
NLM ID
100883448
PMCID
PMC4250751
Subset
IM
External Links
PubMed source DOI: 10.3748/wjg.v11.i5.737 PMC Full Text View journal details
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