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PMID: 15629933 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Evolutionary genetics of a new pathogenic Escherichia species: Escherichia albertii and related Shigella boydii strains.

Journal of bacteriology ·Vol. 187 ·No. 2 ·2005-01-00 ·Pages 619-28

Hyma KE, Lacher DW, Nelson AM, Bumbaugh AC, Janda JM, Strockbine NA, Young VB, Whittam TS

Abstract

A bacterium originally described as Hafnia alvei induces diarrhea in rabbits and causes epithelial damage similar to the attachment and effacement associated with enteropathogenic Escherichia coli. Subsequent studies identified similar H. alvei-like strains that are positive for an intimin gene (eae) probe and, based on DNA relatedness, are classified as a distinct Escherichia species, Escherichia albertii. We determined sequences for multiple housekeeping genes in five E. albertii strains and compared these sequences to those of strains representing the major groups of pathogenic E. coli and Shigella. A comparison of 2,484 codon positions in 14 genes revealed that E. albertii strains differ, on average, at approximately 7.4% of the nucleotide sites from pathogenic E. coli strains and at 15.7% from Salmonella enterica serotype Typhimurium. Interestingly, E. albertii strains were found to be closely related to strains of Shigella boydii serotype 13 (Shigella B13), a distant relative of E. coli representing a divergent lineage in the genus Escherichia. Analysis of homologues of intimin (eae) revealed that the central conserved domains are similar in E. albertii and Shigella B13 and distinct from those of eae variants found in pathogenic E. coli. Sequence analysis of the cytolethal distending toxin gene cluster (cdt) also disclosed three allelic groups corresponding to E. albertii, Shigella B13, and a nontypeable isolate serologically related to S. boydii serotype 7. Based on the synonymous substitution rate, the E. albertii-Shigella B13 lineage is estimated to have split from an E. coli-like ancestor approximately 28 million years ago and formed a distinct evolutionary branch of enteric pathogens that has radiated into groups with distinct virulence properties.

MeSH Terms
Adhesins, Bacterial/genetics Amino Acid Sequence Animals Bacterial Toxins/genetics,toxicity Cell Line DNA, Bacterial/chemistry,isolation & purification Diarrhea/microbiology Enterobacteriaceae Infections/microbiology Escherichia/classification,genetics,isolation & purification,pathogenicity Escherichia coli/genetics Escherichia coli Proteins/genetics Evolution, Molecular Hafnia alvei/classification,genetics,pathogenicity Humans Molecular Sequence Data Phylogeny Rabbits Salmonella typhimurium/genetics Sequence Alignment Sequence Analysis, DNA Sequence Homology Shigella boydii/genetics Virulence
Chemicals
Adhesins, Bacterial Bacterial Toxins DNA, Bacterial Escherichia coli Proteins cytolethal distending toxin eaeA protein, E coli
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Hyma Katie E
Microbial Evolution Laboratory, 165 Food Safety & Toxicology Building, Michigan State University, East Lansing, MI 48824, USA.
Lacher David W
Nelson Adam M
Bumbaugh Alyssa C
Janda J Michael
Strockbine Nancy A
Young Vincent B
Whittam Thomas S
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.187.2.619-628.2005
Published
2005-01-00
Pages
619-28
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC543563
Subset
IM
Grants
NIAID NIH HHS · N01AI30058 · United States
NIAID NIH HHS · N01-AI-30058 · United States
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PubMed source DOI: 10.1128/JB.187.2.619-628.2005 PMC Full Text View journal details
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