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PMID: 15629776 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

IL-15-independent antiviral function of primary and memory CD8+ T cells.

Virology ·Vol. 331 ·No. 2 ·2005-01-20 ·Pages 338-48

Zuo J, Stohlman SA, Bergmann CC

Abstract

Memory CD8+ T cells are comprised of CD122hi IL-15-dependent and CD122lo IL-15-independent subsets. Induction and retention of IL-15-independent memory CD8+ T cells was assessed in IL-15-/- and wild-type (wt) mice immunized with recombinant vaccinia virus (rVV) or Sindbis virus (rSIN) vectors expressing the identical foreign epitope. Both vectors induced epitope-specific CD8+ T cell expansion and function, independent of IL-15. Similar kinetics of rVV clearance confirmed effective CD8+ T cell function in IL-15-/- mice. CD44hi CD122hi CD8+ T cells, mainly of the CD62L-/lo phenotype, increased more dramatically and declined more rapidly in IL-15-/- mice, independent of the vector. Rapid IL-15-independent memory CD8+ T cell expansion following challenge of immune mice compensated for the limited memory CD8+ populations in IL-15-/- mice. However, despite expansion and expression of potent effector function, viral clearance was delayed in the absence of IL-15, coinciding with a rapid loss in cytolytic function.

MeSH Terms
Animals CD8-Positive T-Lymphocytes/immunology Epitopes/immunology Epitopes, T-Lymphocyte/genetics,immunology Genetic Vectors/administration & dosage,genetics,immunology,physiology Immunologic Memory/immunology Interleukin-15/deficiency,immunology Lymphocyte Activation/immunology Mice Mice, Inbred BALB C Sindbis Virus/genetics,immunology,physiology Vaccines, Synthetic/administration & dosage,genetics,immunology Vaccinia virus/genetics,immunology,physiology
Chemicals
Epitopes Epitopes, T-Lymphocyte Interleukin-15 Vaccines, Synthetic
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Zuo Jun
Department of Pathology, University of Southern California Keck School of Medicine, Los Angeles, CA 90033, USA.
Stohlman Stephen A
Bergmann Cornelia C
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Article Info
Journal
Virology
Abbr.
Virology
ISSN
0042-6822
DOI
10.1016/j.virol.2004.10.035
Published
2005-01-20
Pages
338-48
Language
English
Region
United States
NLM ID
0110674
PMCID
PMC7111818
Subset
IM
Grants
NINDS NIH HHS · P01 NS018146 · United States
NINDS NIH HHS · NS 18146 · United States
NIAID NIH HHS · AI 33314 · United States
External Links
PubMed source DOI: 10.1016/j.virol.2004.10.035 PMC Full Text View journal details
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