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PMID: 15612631 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Phylogenetic analysis of bacterial communities associated with larvae of the Atlantic halibut propose succession from a uniform normal flora.

Systematic and applied microbiology ·Vol. 27 ·No. 6 ·2004-11-00 ·Pages 728-36

Jensen S, Ovreås L, Bergh O, Torsvik V

Abstract

Halibut, the largest of all flatfishes is a valuable species with a great potential for aquaculture. Bacteria play an important role in regulating the health of the early life stages. The present article is the first broad-range molecular analysis of bacterial communities in larvae of the Atlantic halibut (Hippoglossus hippoglossus). DNA was extracted from larvae, water and silo biofilm from hatcheries in Norway, Scotland, Iceland and Canada. Eubacterial 16S rRNA gene fragments were amplified by polymerase chain reaction (PCR) with broad-range primers. Sequences spanning the hyper variable V3 region representing individual bacterial species were separated into community profiles by denaturing gradient gel electrophoresis (DGGE). The profiles revealed simple communities after hatching and bacterial succession following growth. Sequencing and phylogenetic analysis of excised DGGE bands suggested aerobic heterotrophs related to groups of Pseudomonas, Janthinobacterium and possibly Marinomonas to be the primary colonisers of the larvae. After onset of feeding, fermentative species (Vibrio) were detected as well. Comparative analysis of bacterial communities from different geographical regions indicated that larvae of the Atlantic halibut possess a distinct and specific normal flora.

MeSH Terms

Animals Bacteria/classification,isolation & purification Biofilms Canada DNA Fingerprinting DNA, Bacterial/analysis,chemistry,isolation & purification DNA, Ribosomal/chemistry,isolation & purification Flounder/microbiology Genes, rRNA Iceland Larva/microbiology Molecular Sequence Data Norway Nucleic Acid Denaturation Oceanospirillaceae/classification,isolation & purification Oxalobacteraceae/classification,isolation & purification Phylogeny Pseudomonas/classification,isolation & purification RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics Scotland Seawater/microbiology Sequence Analysis, DNA Vibrio/classification,isolation & purification Water Microbiology

Chemicals

DNA, Bacterial DNA, Ribosomal RNA, Bacterial RNA, Ribosomal, 16S

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Jensen Sigmund

Department of Aquaculture, Institute of Marine Research, Nordnes, Bergen, Norway. sigmund.jensen@imr.no

Ovreås Lise

Bergh Oivind

Torsvik Vigdis

Article Info

Journal

Systematic and applied microbiology

Abbr.

Syst Appl Microbiol

ISSN

0723-2020

DOI

10.1078/0723202042369929

sigmund.jensen@imr.no

Published

2004-11-00

Pages

728-36

Language

English

Region

Germany

NLM ID

8306133

Subset

Databases

GENBANK

AY618623, AY618624, AY618625, AY618626, AY618627, AY618628, AY618629, AY618630, AY618631, AY618632, AY618633, AY618634, AY618635, AY618636, AY618637, AY618638, AY618639, AY618640, AY618641, AY618642, AY618643, AY618644, AY618645, AY618646, AY618647, AY618648, AY618649, AY618650, AY618651, AY618652, AY618653, AY618654

External Links

PubMed source DOI: 10.1078/0723202042369929 View journal details

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Phylogenetic analysis of bacterial communities associated with larvae of the Atlantic halibut propose succession from a uniform normal flora.

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