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PMID: 1560544 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Theiler's virus replication in brain macrophages cultured in vitro.

Journal of virology ·Vol. 66 ·No. 5 ·1992-05-00 ·Pages 3188-93

Levy M, Aubert C, Brahic M

Abstract

Infection of the mouse with Theiler's virus is one of the best animal models for the study of multiple sclerosis, a chronic demyelinating disease of the human central nervous system. The identification of the virus target cell(s) is fundamental to an understanding of the viral persistence as well as the inflammation and demyelination observed in the chronic phase of the disease. This paper reports that a small fraction of brain macrophages grown in vitro can be efficiently infected with Theiler's virus without significant cytolytic effect. Viral replication as well as continuous production of infectivity were observed in these cultures.

MeSH Terms
Animals Brain/microbiology Cells, Cultured Disease Models, Animal Enterovirus Infections/pathology Histocytochemistry Macrophages/microbiology Maus Elberfeld virus/growth & development Mice Nucleic Acid Hybridization RNA, Viral/isolation & purification Virus Replication
Chemicals
RNA, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Levy M
Unité des Virus Lents, UA 1157 Centre National de la Recherche Scientifique, Institut Pasteur, Paris, France.
Aubert C
Brahic M
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.66.5.3188-3193.1992
Published
1992-05-00
Pages
3188-93
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC241085
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.66.5.3188-3193.1992 PMC Full Text View journal details
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