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PMID: 15601896 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The FIP3-Rab11 protein complex regulates recycling endosome targeting to the cleavage furrow during late cytokinesis.

Molecular biology of the cell ·Vol. 16 ·No. 2 ·2005-02-00 ·Pages 849-60

Wilson GM, Fielding AB, Simon GC, Yu X, Andrews PD, Hames RS, Frey AM, Peden AA, Gould GW, Prekeris R

Abstract

An integral part of cell division is the separation of daughter cells via cytokinesis. There is now good evidence that the completion of cytokinesis requires coordinated membrane trafficking to deliver new membrane to the tip of the furrow and to complete the abscission. Here we have examined membrane traffic in cytokinesis and describe several novel observations. First, we show that Rab11- and FIP3-containing recycling endosomes accumulate near the cleavage furrow and are required for successful completion of cytokinesis. Second, we demonstrate that the Rab11-FIP3 protein complex is intimately involved in the delivery of endosomes to the cleavage furrow. Significantly, although FIP3 recruitment to endosomes is Rab11 dependent, we find that the targeting of FIP3 to the midbody is independent of Rab11. Third, we show that the Rab11-FIP3 complex is required for a late stage of cytokinesis, possibly abscission. Finally, we demonstrate that localization of FIP3 is subject to substantial spatial and temporal regulation. These data provide the first detailed analysis of recycling endosomes in cell division and provide a new model for membrane traffic to the furrow. We propose that the dynamic Rab11-FIP3 interaction controls the delivery, targeting, and fusion of recycling endosomes with furrow during late cytokinesis and abscission.

MeSH Terms
Animals Carrier Proteins/metabolism Cell Cycle Cell Membrane/metabolism Cytokinesis DNA-Binding Proteins/metabolism Endosomes/metabolism Flow Cytometry Fluorescence Resonance Energy Transfer HeLa Cells Humans I-kappa B Kinase Mice Microscopy, Confocal Microscopy, Video NIH 3T3 Cells Protein Serine-Threonine Kinases/metabolism Protein Transport RNA Interference Time Factors Transcription Factors/metabolism Upstream Stimulatory Factors rab GTP-Binding Proteins/metabolism
Chemicals
Carrier Proteins DNA-Binding Proteins IKBKG protein, human Transcription Factors Upstream Stimulatory Factors Protein Serine-Threonine Kinases CHUK protein, human Chuk protein, mouse I-kappa B Kinase IKBKB protein, human IKBKE protein, human Ikbkb protein, mouse Ikbke protein, mouse rab11 protein rab GTP-Binding Proteins
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Wilson Gayle M
Department of Cellular and Developmental Biology, School of Medicine, University of Colorado Health Sciences Center, Denver, CO 80262, USA.
Fielding Andrew B
Simon Glenn C
Yu Xinzi
Andrews Paul D
Hames Rebecca S
Frey Andrew M
Peden Andrew A
Gould Gwyn W
Prekeris Rytis
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e04-10-0927
Published
2005-02-00
Epub
2004-00-15
Pages
849-60
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC545916
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.e04-10-0927 PMC Full Text View journal details
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