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PMID: 15591206 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The Gs-linked receptor GPR3 maintains meiotic arrest in mammalian oocytes.

Science (New York, N.Y.) ·Vol. 306 ·No. 5703 ·2004-12-10 ·Pages 1947-50

Mehlmann LM, Saeki Y, Tanaka S, Brennan TJ, Evsikov AV, Pendola FL, Knowles BB, Eppig JJ, Jaffe LA

Abstract

Mammalian oocytes are held in prophase arrest by an unknown signal from the surrounding somatic cells. Here we show that the orphan Gs-linked receptor GPR3, which is localized in the oocyte, maintains this arrest. Oocytes from Gpr3 knockout mice resume meiosis within antral follicles, independently of an increase in luteinizing hormone, and this phenotype can be reversed by injection of Gpr3 RNA into the oocytes. Thus, the GPR3 receptor is a link in communication between the somatic cells and oocyte of the ovarian follicle and is crucial for the regulation of meiosis.

MeSH Terms

Adenylyl Cyclases/metabolism Animals Chondroitin Sulfate Proteoglycans/genetics,metabolism Expressed Sequence Tags Female Granulosa Cells/physiology Heterotrimeric GTP-Binding Proteins/metabolism In Situ Hybridization Lectins, C-Type Ligands Luteinizing Hormone/metabolism Meiosis Metaphase Mice Mice, Inbred C57BL Mice, Knockout Mitosis Oocytes/physiology Ovarian Follicle/physiology RNA/genetics,metabolism Receptors, G-Protein-Coupled/genetics,physiology Versicans

Chemicals

Chondroitin Sulfate Proteoglycans GPR3 protein, mouse Lectins, C-Type Ligands Receptors, G-Protein-Coupled Vcan protein, mouse Versicans RNA Luteinizing Hormone Heterotrimeric GTP-Binding Proteins Adenylyl Cyclases

Authors & Affiliations

9 authors, click to expand affiliations / ORCID

Mehlmann Lisa M

Department of Cell Biology, University of Connecticut Health Center (UCHC), Farmington, CT 06032, USA. lmehlmann@neuron.uchc.edu

Saeki Yoshinaga

Tanaka Shigeru

Brennan Thomas J

Evsikov Alexei V

Pendola Frank L

Knowles Barbara B

Eppig John J

Jaffe Laurinda A

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

1095-9203

DOI

10.1126/science.1103974

lmehlmann@neuron.uchc.edu

Published

2004-12-10

Pages

1947-50

Language

English

Region

United States

NLM ID

0404511

Subset

External Links

PubMed source DOI: 10.1126/science.1103974 View journal details

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The Gs-linked receptor GPR3 maintains meiotic arrest in mammalian oocytes.

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