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PMID: 15579440 Published · ppublish English Journal Article

Expression of stromal cell-derived factor-1 and of its receptor CXCR4 in liver regeneration from oval cells in rat.

The American journal of pathology ·Vol. 165 ·No. 6 ·2004-12-00 ·Pages 1969-77

Mavier P, Martin N, Couchie D, Préaux AM, Laperche Y, Zafrani ES

Abstract

Stromal cell-derived factor-1 is a chemokine that plays a major role during embryogenesis. Since stromal cell-derived factor-1 and its unique receptor CXCR4 are involved in the differentiation of progenitor cells, we studied the expression of this chemokine and of its receptor in hepatic regeneration from precursor oval cells. Hepatic regeneration was induced by treating rats with 2-acetylaminofluorene, and followed by partial hepatectomy. Oval cell accumulation, which predominated in periportal regions, reached a maximum at days 9 to 14 after hepatectomy and declined thereafter. Oval cells strongly expressed stromal cell-derived factor-1 protein and mRNA. CXCR4 mRNA hepatic level paralleled the number of oval cells and in situ hybridization showed CXCR4 mRNA expression by these cells. Treatment of rats with fucoidan, a sulfated polysaccharide which binds to stromal cell-derived factor-1 and blocks its biological effects, markedly decreased oval cell accumulation in five of the seven treated rats. In conclusion, our data demonstrate an expression of stromal cell-derived factor-1 and of its receptor CXCR4 in oval cells during hepatic regeneration and strongly suggest that stromal cell-derived factor-1 stimulates the proliferation of these precursor cells through an autocrine/paracrine pathway.

MeSH Terms
2-Acetylaminofluorene/pharmacology Animals Chemokine CXCL12 Chemokines, CXC/genetics,metabolism Hepatectomy Liver/cytology,drug effects,metabolism Liver Regeneration Male Polysaccharides/metabolism RNA, Messenger/genetics,metabolism Rats Rats, Inbred F344 Rats, Wistar Receptors, CXCR4/genetics,metabolism Reverse Transcriptase Polymerase Chain Reaction Stromal Cells/metabolism
Chemicals
Chemokine CXCL12 Chemokines, CXC Polysaccharides RNA, Messenger Receptors, CXCR4 fucoidan 2-Acetylaminofluorene
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Mavier Philippe
INSERM U581, Hôpital Henri Mondor, Université Paris XII-Val de Marne, 94010 Créteil, France. mavier@im3.inserm.fr
Martin Nadine
Couchie Dominique
Préaux Anne-Marie
Laperche Yannick
Zafrani Elie Serge
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
DOI
10.1016/S0002-9440(10)63248-8
Email
mavier@im3.inserm.fr
Published
2004-12-00
Pages
1969-77
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1618713
Subset
IM
External Links
PubMed source DOI: 10.1016/S0002-9440(10)63248-8 PMC Full Text View journal details
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