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PMID: 15576799 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Regulation of sigmaB by an anti- and an anti-anti-sigma factor in Streptomyces coelicolor in response to osmotic stress.

Journal of bacteriology ·Vol. 186 ·No. 24 ·2004-12-00 ·Pages 8490-8

Lee EJ, Cho YH, Kim HS, Ahn BE, Roe JH

Abstract

sigmaB, a homolog of stress-responsive sigmaB of Bacillus subtilis, controls both osmoprotection and differentiation in Streptomyces coelicolor A3 (2). Its gene is preceded by rsbA and rsbB genes encoding homologs of an anti-sigma factor, RsbW, and its antagonist, RsbV, of B. subtilis, respectively. Purified RsbA bound to sigmaB and prevented sigmaB-directed transcription from the sigBp1 promoter in vitro. An rsbA-null mutant exhibited contrasting behavior to the sigB mutant, with elevated sigBp1 transcription, no actinorhodin production, and precocious aerial mycelial formation, reflecting enhanced activity of sigmaB in vivo. Despite sequence similarity to RsbV, RsbB lacks the conserved phosphorylatable serine residue and its gene disruption produced no distinct phenotype. RsbV (SCO7325) from a putative six-gene operon (rsbV-rsbR-rsbS-rsbT-rsbU1-rsbU) was strongly induced by osmotic stress in a sigmaB-dependent manner. It antagonized the inhibitory action of RsbA on sigmaB-directed transcription and was phosphorylated by RsbA in vitro. These results support the hypothesis that the rapid induction of sigmaB target genes by osmotic stress results from modulation of sigmaB activity by the kinase-anti-sigma factor RsbA and its phosphorylatable antagonist RsbV, which function by a partner-switching mechanism. Amplified induction could result from a rapid increase in the synthesis of both sigmaB and its inhibitor antagonist.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/antagonists & inhibitors,chemistry,genetics,metabolism Base Sequence Carrier Proteins/chemistry,genetics,metabolism Culture Media Gene Expression Regulation, Bacterial Molecular Sequence Data Osmotic Pressure Phosphorylation Repressor Proteins/chemistry,genetics,metabolism Sigma Factor/antagonists & inhibitors,chemistry,genetics,metabolism Streptomyces coelicolor/genetics,metabolism,physiology
Chemicals
Bacterial Proteins Carrier Proteins Culture Media RSBV protein, Streptomyces coelicolor Repressor Proteins RsbW protein, bacteria SigB protein, Bacteria Sigma Factor
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lee Eun-Jin
School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul 151-742, Korea.
Cho You-Hee
Kim Hyo-Sub
Ahn Bo-Eun
Roe Jung-Hye
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.186.24.8490-8498.2004
Published
2004-12-00
Pages
8490-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC532406
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.186.24.8490-8498.2004 PMC Full Text View journal details
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