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PMID: 15557173 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Antibody repertoire development in fetal and neonatal pigs. VII. Characterization of the preimmune kappa light chain repertoire.

Journal of immunology (Baltimore, Md. : 1950) ·Vol. 173 ·No. 11 ·2004-12-01 ·Pages 6794-805

Butler JE, Wertz N, Sun J, Wang H, Chardon P, Piumi F, Wells K

Abstract

Combinatorial diversity is highly restricted in the preimmune porcine H chain repertoire compared with that in humans and mice. This raised the question of whether similar restriction characterized the preimmune L chain repertoire. In this study we present evidence that >90% of all expressed Vkappa genes in the porcine preimmune repertoire belong to three subfamilies of Vkappa genes that share 87% sequence similarity with human IGKV2. This porcine Vkappa family also shares sequence similarity with some, but not all, Vkappa genes from sheep. Hybridization with sperm DNA and sequence analyses of polynucleotides from overlapping bacterial artificial chromosome clones suggest swine possess approximately 60 IGVK2 genes. The latter method also revealed that certain IGKV2 subfamilies are not expressed in the preimmune repertoire. Six members of an IGVK1 family were also expressed as part of the preimmune repertoire, and these shared 87% sequence similarity with human IGVK1. Five Jkappa segments, complete with recombination signal sequences and separated by approximately 300 nt, were identified approximately 3 kb upstream of a single Ckappa. Surprisingly, Jkappa2 accounted for >90% of all framework region 4 sequences in the preimmune repertoire. These findings show that swine use approximately 10 IGVK2 genes from three of six subfamilies and preferentially one Jkappa segment to generate their preimmune kappa repertoire. These studies, like those of porcine Ig constant regions and MHC genes, also indicate unexpected high sequence similarity with their human counterparts despite differences in phylogeny and the mechanism of repertoire diversification.

MeSH Terms

Amino Acid Motifs/genetics,immunology Amino Acid Sequence Animals Animals, Newborn/genetics,immunology Antibody Diversity/genetics Base Sequence Chromosomes, Artificial, Bacterial Cloning, Molecular/methods Fetal Development/genetics,immunology Gene Expression Regulation, Developmental/immunology Gene Rearrangement, B-Lymphocyte, Light Chain Genes, Overlapping Genome Humans Immunoglobulin J-Chains/biosynthesis,chemistry,genetics Immunoglobulin Variable Region/biosynthesis,chemistry,genetics Immunoglobulin kappa-Chains/biosynthesis,chemistry,genetics Male Molecular Sequence Data Multigene Family/immunology Sequence Alignment Sequence Homology, Nucleic Acid Swine Terminology as Topic

Chemicals

Immunoglobulin J-Chains Immunoglobulin Variable Region Immunoglobulin kappa-Chains

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Butler John E

Department of Microbiology and Interdisciplinary Immunology Program, University of Iowa, Iowa City, IA 52242, USA. john-butler@uiowa.edu

Wertz Nancy

Sun Jishan

Wang Huang

Chardon Patrick

Piumi Francois

Wells Kevin

Article Info

Journal

Journal of immunology (Baltimore, Md. : 1950)

Abbr.

J Immunol

ISSN

0022-1767

DOI

10.4049/jimmunol.173.11.6794

john-butler@uiowa.edu

Published

2004-12-01

Pages

6794-805

Language

English

Region

United States

NLM ID

2985117R

Subset

Databases

GENBANK

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External Links

PubMed source DOI: 10.4049/jimmunol.173.11.6794 View journal details

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Antibody repertoire development in fetal and neonatal pigs. VII. Characterization of the preimmune kappa light chain repertoire.

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