Abstract
In hepatocyte cultures, insulin stimulates cellular accumulation of K+, partly (approximately 20%) by net replacement of cell Na+, but largely (approximately 80%) by increasing the cell K++Na+ content, with a consequent increase in cell volume. An increase in cation content occurred within 5 min of exposure to insulin and was not secondary to metabolic changes. Insulin also increased the cation content, by increasing the Na+ content, in a K(+)-free medium or when K+ uptake was inhibited with 1 mM-ouabain. However, insulin did not increase the cation content in a Na(+)-free medium. The stimulation of glycogen synthesis by insulin, like the increase in cation content, was blocked in a Na(+)-free medium, but not when K+ uptake was inhibited. Hypo-osmotic swelling restored the stimulation of glycogen synthesis in a Na(+)-free medium, indicating that the lack of effect of insulin in the iso-osmotic Na(+)-free medium was not due to a direct requirement for Na+ for glycogen synthesis, but to a secondary mechanism, dependent on Na+ entry, that can be mimicked by hypo-osmotic swelling. Quinine increased cell volume further and caused a further increase in glycogen synthesis. The hypothesis that cellular uptake of K+ may be part of the mechanism by which insulin controls metabolism was discounted, because inhibition of K+ uptake does not block the metabolic effects of insulin [Czech (1977) Annu. Rev. Biochem. 46, 359-384]. The present results support the hypothesis that an increase in cell cation content, and thereby cell volume, rather than K+ uptake, is part of the mechanism by which insulin stimulates glycogen synthesis in hepatocytes.
MeSH Terms
Amino Acids/pharmacology
Animals
Cells, Cultured
Chlorides/metabolism
Culture Media
Glycogen Synthase/drug effects,metabolism
Hypotonic Solutions/pharmacology
Insulin/pharmacology
Liver/cytology,drug effects,metabolism
Liver Glycogen/biosynthesis
Male
Osmolar Concentration
Ouabain/pharmacology
Potassium/metabolism
Quinine/pharmacology
Rats
Rats, Inbred Strains
Sodium/metabolism
Stimulation, Chemical
Chemicals
Amino Acids
Chlorides
Culture Media
Hypotonic Solutions
Insulin
Liver Glycogen
Ouabain
Sodium
Quinine
Glycogen Synthase
Potassium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
al-Habori M
Department of Medicine, Medical School, University of Newcastle upon Tyne, U.K.
Peak M
Thomas T H
Agius L
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