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PMID: 15542640 Published · ppublish English Journal Article

Recombinant human Metapneumovirus lacking the small hydrophobic SH and/or attachment G glycoprotein: deletion of G yields a promising vaccine candidate.

Journal of virology ·Vol. 78 ·No. 23 ·2004-12-00 ·Pages 12877-87

Biacchesi S, Skiadopoulos MH, Yang L, Lamirande EW, Tran KC, Murphy BR, Collins PL, Buchholz UJ

Abstract

Human metapneumovirus (HMPV) has recently been identified as a significant cause of serious respiratory tract disease in humans. In particular, the emerging information on the contribution of HMPV to pediatric respiratory tract disease suggests that it will be important to develop a vaccine against this virus for use in conjunction with those being developed for human respiratory syncytial virus and the human parainfluenza viruses. A recently described reverse genetic system (S. Biacchesi, M. H. Skiadopoulos, K. C. Tran, B. R. Murphy, P. L. Collins, and U. J. Buchholz, Virology 321:247-259, 2004) was used to generate recombinant HMPVs (rHMPVs) that lack the G gene, the SH gene, or both. The DeltaSH, DeltaG, and DeltaSH/G deletion mutants were readily recovered and were found to replicate efficiently during multicycle growth in cell culture. Thus, the SH and G proteins are not essential for growth in cell culture. Apart from the absence of the deleted protein(s), the virions produced by the gene deletion mutants were similar by protein yield and gel electrophoresis protein profile to wild-type HMPV. When administered intranasally to hamsters, the DeltaG and DeltaSH/G mutants replicated in both the upper and lower respiratory tracts, showing that HMPV containing F as the sole viral surface protein is competent for replication in vivo. However, both viruses were at least 40-fold and 600-fold restricted in replication in the lower and upper respiratory tract, respectively, compared to wild-type rHMPV. They also induced high titers of HMPV-neutralizing serum antibodies and conferred complete protection against replication of wild-type HMPV challenge virus in the lungs. Surprisingly, G is dispensable for protection, and the DeltaG and DeltaSH/G viruses represent promising vaccine candidates. In contrast, DeltaSH replicated somewhat more efficiently in hamster lungs compared to wild-type rHMPV (20-fold increase on day 5 postinfection). This indicates that SH is completely dispensable in vivo and that its deletion does not confer an attenuating effect, at least in this rodent model.

MeSH Terms
Animals Cricetinae Gene Deletion HN Protein/genetics,physiology Mesocricetus Metapneumovirus/immunology,physiology RNA, Viral/biosynthesis Recombination, Genetic Viral Envelope Proteins Viral Proteins/physiology Viral Vaccines/immunology Virus Replication
Chemicals
HN Protein RNA, Viral Viral Envelope Proteins Viral Proteins Viral Vaccines attachment protein G
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Biacchesi Stéphane
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892-8007, USA.
Skiadopoulos Mario H
Yang Lijuan
Lamirande Elaine W
Tran Kim C
Murphy Brian R
Collins Peter L
Buchholz Ursula J
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
Published
2004-12-00
Pages
12877-87
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC525014
Subset
IM
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