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PMID: 15542490 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Organization of monoterpene biosynthesis in Mentha. Immunocytochemical localizations of geranyl diphosphate synthase, limonene-6-hydroxylase, isopiperitenol dehydrogenase, and pulegone reductase.

Plant physiology ·Vol. 136 ·No. 4 ·2004-12-00 ·Pages 4215-27

Turner GW, Croteau R

Abstract

We present immunocytochemical localizations of four enzymes involved in p-menthane monoterpene biosynthesis in mint: the large and small subunits of peppermint (Mentha x piperita) geranyl diphosphate synthase, spearmint (Mentha spicata) (-)-(4S)-limonene-6-hydroxylase, peppermint (-)-trans-isopiperitenol dehydrogenase, and peppermint (+)-pulegone reductase. All were localized to the secretory cells of peltate glandular trichomes with abundant labeling corresponding to the secretory phase of gland development. Immunogold labeling of geranyl diphosphate synthase occurred within secretory cell leucoplasts, (-)-4S-limonene-6-hydroxylase labeling was associated with gland cell endoplasmic reticulum, (-)-trans-isopiperitenol dehydrogenase labeling was restricted to secretory cell mitochondria, while (+)-pulegone reductase labeling occurred only in secretory cell cytoplasm. We discuss this pathway compartmentalization in relation to possible mechanisms for the intracellular movement of monoterpene metabolites, and for monoterpene secretion into the extracellular essential oil storage cavity.

MeSH Terms
Alcohol Oxidoreductases/analysis Alkyl and Aryl Transferases/analysis Cytochrome P-450 Enzyme System/analysis Farnesyltranstransferase Gene Expression Regulation, Plant Immunohistochemistry Mentha/enzymology,metabolism,ultrastructure Mixed Function Oxygenases/analysis Monoterpenes/metabolism NAD (+) and NADP (+) Dependent Alcohol Oxidoreductases Oxidoreductases/analysis Plant Proteins Subcellular Fractions/enzymology
Chemicals
Monoterpenes Plant Proteins Cytochrome P-450 Enzyme System Mixed Function Oxygenases Oxidoreductases Alcohol Oxidoreductases NAD (+) and NADP (+) Dependent Alcohol Oxidoreductases isopiperitenol dehydrogenase limonene-6-hydroxylase pulegone reductase Alkyl and Aryl Transferases Farnesyltranstransferase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Turner Glenn W
Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164, USA.
Croteau Rodney
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.104.050229
Published
2004-12-00
Epub
2004-00-12
Pages
4215-27
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC535851
Subset
IM
External Links
PubMed source DOI: 10.1104/pp.104.050229 PMC Full Text View journal details
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