Home LiteratureArticle Details

PMID: 15525538 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

ACAP1 promotes endocytic recycling by recognizing recycling sorting signals.

Developmental cell ·Vol. 7 ·No. 5 ·2004-11-00 ·Pages 771-6

Dai J, Li J, Bos E, Porcionatto M, Premont RT, Bourgoin S, Peters PJ, Hsu VW

Abstract

Cargo sorting that promotes the transport of cargo proteins from a membrane compartment has been predicted to be unlikely in the endocytic recycling pathways. We now show that ACAP1 binds specifically and directly to recycling cargo proteins. Reducing this interaction for TfR inhibits its recycling. Moreover, ACAP1 binds to two distinct phenylalanine-based sequences in the cytoplasmic domain of TfR that function as recycling sorting signals to promote its transport from the recycling endosome. Taken together, these findings indicate that ACAP1 promotes cargo sorting by recognizing recycling sorting signals.

MeSH Terms

ADP-Ribosylation Factor 6 ADP-Ribosylation Factors/genetics,metabolism Alanine/metabolism Amino Acid Sequence Amino Acid Substitution Animals Blotting, Western CHO Cells Carrier Proteins/metabolism Cricetinae Cricetulus Endocytosis Fluorescent Dyes GTPase-Activating Proteins/genetics,metabolism Glutathione Transferase/metabolism Green Fluorescent Proteins/metabolism HeLa Cells Humans Microscopy, Fluorescence Organic Chemicals Point Mutation Precipitin Tests Protein Sorting Signals Protein Structure, Tertiary RNA, Small Interfering/metabolism Receptors, Transferrin/chemistry Recombinant Fusion Proteins/metabolism

Chemicals

ADP-Ribosylation Factor 6 Alexa594 Carrier Proteins Fluorescent Dyes GTPase-Activating Proteins Organic Chemicals Protein Sorting Signals RNA, Small Interfering Receptors, Transferrin Recombinant Fusion Proteins Green Fluorescent Proteins Glutathione Transferase ADP-Ribosylation Factors Alanine

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Dai Jun

Division of Rheumatology, Immunology, and Allergy, Brigham and Women's Hospital, Department of Medicine, Harvard Medical School, Boston, MA 02115, USA.

Li Jian

Bos Erik

Porcionatto Marimelia

Premont Richard T

Bourgoin Sylvain

Peters Peter J

Hsu Victor W

Article Info

Journal

Developmental cell

Abbr.

Dev Cell

ISSN

1534-5807

DOI

10.1016/j.devcel.2004.10.002

Published

2004-11-00

Pages

771-6

Language

English

Region

United States

NLM ID

101120028

Subset

External Links

PubMed source DOI: 10.1016/j.devcel.2004.10.002 View journal details

Analysis Services

ACAP1 promotes endocytic recycling by recognizing recycling sorting signals.

Contact

WeChat Official Account