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PMID: 15504329 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Seizure-induced plasticity of h channels in entorhinal cortical layer III pyramidal neurons.

Neuron ·Vol. 44 ·No. 3 ·2004-10-28 ·Pages 495-508

Shah MM, Anderson AE, Leung V, Lin X, Johnston D

Abstract

The entorhinal cortex (EC) provides the predominant excitatory drive to the hippocampal CA1 and subicular neurons in chronic epilepsy. Discerning the mechanisms underlying signal integration within EC neurons is essential for understanding network excitability alterations involving the hippocampus during epilepsy. Twenty-four hours following a single seizure episode when there were no behavioral or electrographic seizures, we found enhanced spontaneous activity still present in the rat EC in vivo and in vitro. The increased excitability was accompanied by a profound reduction in I(h) in EC layer III neurons and a significant decline in HCN1 and HCN2 subunits that encode for h channels. Consequently, dendritic excitability was enhanced, resulting in increased neuronal firing despite hyperpolarized membrane potentials. The loss of I(h) and the increased neuronal excitability persisted for 1 week following seizures. Our results suggest that dendritic I(h) plays an important role in determining the excitability of EC layer III neurons and their associated neural networks.

MeSH Terms
Analysis of Variance Animals Bicuculline/pharmacology Blotting, Western/methods Cyclic Nucleotide-Gated Cation Channels Dendrites/drug effects,physiology Electroencephalography/methods Entorhinal Cortex/pathology Excitatory Amino Acid Agonists/pharmacology Excitatory Postsynaptic Potentials/drug effects,physiology GABA Antagonists/pharmacology Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels Immunohistochemistry/methods In Vitro Techniques Ion Channels/antagonists & inhibitors,physiology Kainic Acid Male Membrane Potentials/drug effects,physiology,radiation effects Muscle Proteins/physiology Neuronal Plasticity/drug effects,physiology Patch-Clamp Techniques/methods Potassium Channels Pyramidal Cells/drug effects,physiopathology Pyrimidines/pharmacology Rats Rats, Sprague-Dawley Seizures/chemically induced,physiopathology Valine/analogs & derivatives,pharmacology
Chemicals
Cyclic Nucleotide-Gated Cation Channels Excitatory Amino Acid Agonists GABA Antagonists Hcn1 protein, rat Hcn2 protein, rat Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels Ion Channels Muscle Proteins Potassium Channels Pyrimidines ICI D2788 2-amino-5-phosphopentanoic acid Valine Kainic Acid Bicuculline
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Shah Mala M
Division of Neuroscience, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA.
Anderson Anne E
Leung Victor
Lin Xiaodi
Johnston Daniel
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Article Info
Journal
Neuron
Abbr.
Neuron
ISSN
0896-6273
DOI
10.1016/j.neuron.2004.10.011
Published
2004-10-28
Pages
495-508
Language
English
Region
United States
NLM ID
8809320
PMCID
PMC2386958
Subset
IM
Grants
Wellcome Trust · 063247 · United Kingdom
Corrections
CommentIn
PMID: 16145611
External Links
PubMed source DOI: 10.1016/j.neuron.2004.10.011 PMC Full Text View journal details
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