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题目:: Proliferation potential-related protein, an ideal esophageal cancer antigen for immunotherapy, identified using complementary DNA microarray analysis.
作者:: Yoshitake(Yoshihiro),Nakatsura(Tetsuya),Monji(Mikio),Senju(Satoru),Matsuyoshi(Hidetake),Tsukamoto(Hirotake),Hosaka(Seiji),Komori(Hiroyuki),Fukuma(Daiki),Ikuta(Yoshiaki),Katagiri(Toyomasa),Furukawa(Yoichi),Ito(Hiromi),Shinohara(Masanori),Nakamura(Yusuke),Nishimura(Yasuharu)
状态:: 发布时间2004-10-11 , 更新时间 2011-11-17
期刊:: Clin Cancer Res
摘要:: To establish effective antitumor immunotherapy for esophageal cancer, we tried to identify an useful target antigen of esophageal cancer.,We did cDNA microarray analysis to find a novel candidate antigen, proliferation potential-related protein (PP-RP). We examined cytotoxicity against tumor cells in vitro and in vivo of CTLs specific to PP-RP established from esophageal cancer patients.,In 26 esophageal cancer tissues, an average of relative ratio of the expression of the PP-RP mRNA in cancer cells versus adjacent normal esophageal tissues was 396.2. Immunohistochemical analysis revealed that, in 20 of the 22 esophageal cancer tissues, PP-RP protein was strongly expressed only in the cancer cells and not so in normal esophageal epithelial cells. PP-RP protein contains 10 epitopes recognized by HLA-A24-restricted CTLs. These CTLs, generated from HLA-A24-positive esophageal cancer patients, had cytotoxic activity against cancer cell lines positive for both PP-RP and HLA-A24. Furthermore, adoptive transfer of the PP-RP-specific CTL line inhibited the growth of a human esophageal cancer cell line engrafted in nude mice.,The expression of PP-RP in esophageal cancer cells was significantly higher than in normal cells, and the CTLs recognizing PP-RP killed tumor cells in vitro and also showed tumor rejection effects in a xenograft model. Therefore, PP-RP may prove to be an ideal tumor antigen useful for diagnosis and immunotherapy for patients with esophageal cancer. cDNA microarray analysis is a useful method to identify ideal tumor-associated antigens.
语言:: eng
DOI:: 10.1158/1078-0432.CCR-04-0841

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