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PMID: 15454461 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Kinetics of intermolecular interaction during protein folding of reduced cytochrome c.

Biophysical journal ·Vol. 87 ·No. 4 ·2004-10-00 ·Pages 2663-75

Nishida S, Nada T, Terazima M

Abstract

Kinetics of intermolecular interaction between reduced cytochrome c (Cyt c) protein and solvent during the protein-refolding process is studied by monitoring the time dependence of apparent diffusion coefficient (D) using the pulsed-laser-induced transient grating technique. The refolding was triggered by photoinduced reduction of unfolded Fe(III) Cyt c in 3.5 M guanidine hydrochloride (GdnHCl) solution and the change in the diffusion coefficient was monitored in time domain. The relationship between D and the protein conformations under equilibrium condition were investigated at various GdnHCl concentrations using a photolabeling reagent. The time dependence of the observed transient grating signal was analyzed using these data and two models: a continuous change model of the intermolecular interaction and a two-state model. It was found that the TG signals in various time ranges can be consistently reproduced well by the two-state model. The dynamics of D is expressed well by a single exponential function with a rate constant of 22 +/- 7 s(-1) in a whole time range. The folding process of Cyt c is discussed based on these observations.

MeSH Terms

Carbon Monoxide/chemistry Computer Simulation Cytochromes c/chemistry Diffusion Elasticity Entropy Enzyme Activation Enzyme Stability Guanidine/chemistry Hydrogen-Ion Concentration Kinetics Macromolecular Substances/chemistry Models, Chemical Oxidation-Reduction Protein Binding Protein Conformation Protein Denaturation Protein Folding Solutions Solvents/chemistry

Chemicals

Macromolecular Substances Solutions Solvents Carbon Monoxide Cytochromes c Guanidine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Nishida Shinpei

Department of Chemistry, Graduate School of Science, Kyoto University, Kyoto, 606-8502, Japan.

Nada Tomokazu

Terazima Masahide

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23 references, click to expand

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Article Info

Journal

Biophysical journal

Abbr.

Biophys J

ISSN

0006-3495

DOI

10.1529/biophysj.104.042531

Published

2004-10-00

Pages

2663-75

Language

English

Region

United States

NLM ID

0370626

PMCID

PMC1304685

Subset

External Links

PubMed source DOI: 10.1529/biophysj.104.042531 PMC Full Text View journal details

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Kinetics of intermolecular interaction during protein folding of reduced cytochrome c.

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