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PMID: 1542645 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A streptavidin-metallothionein chimera that allows specific labeling of biological materials with many different heavy metal ions.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 5 ·1992-03-01 ·Pages 1534-8

Sano T, Glazer AN, Cantor CR

Abstract

We have designed a streptavidin-metallothionein chimeric protein in which the streptavidin moiety provides a means of binding the metallothionein moiety tightly to specific biological targets. A gene fusion of streptavidin with mouse metallothionein I was efficiently expressed in Escherichia coli, and the expressed chimeric protein was purified to homogeneity by a simple procedure. The purified chimera, consisting of four identical subunits, bound one biotin and approximately seven Cd2+ ions per subunit (19.5 kDa). This indicates that both the streptavidin and the metallothionein moieties are fully functional. The high binding affinity of the chimera both for biotin and for heavy metal ions allows the specific labeling or conjugation of any biological material containing unhindered biotin with a variety of different heavy metal ions and their isotopes, thereby opening the way for simultaneous assay systems for a large number of biological targets.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/chemistry Base Sequence Biotin/chemistry Cadmium/chemistry Escherichia coli Metallothionein/chemistry Metals/chemistry Molecular Sequence Data Molecular Weight Recombinant Fusion Proteins/chemistry Streptavidin
Chemicals
Bacterial Proteins Metals Recombinant Fusion Proteins Cadmium Biotin Streptavidin Metallothionein
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sano T
Department of Molecular and Cell Biology, University of California, Berkeley 94720.
Glazer A N
Cantor C R
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.5.1534
Published
1992-03-01
Pages
1534-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC48486
Subset
IM
Grants
NCI NIH HHS · CA39782 · United States
External Links
PubMed source DOI: 10.1073/pnas.89.5.1534 PMC Full Text View journal details
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