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PMID: 15377760 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Divergent roles of a pair of homologous jumonji/zinc-finger-class transcription factor proteins in the regulation of Arabidopsis flowering time.

The Plant cell ·Vol. 16 ·No. 10 ·2004-10-00 ·Pages 2601-13

Noh B, Lee SH, Kim HJ, Yi G, Shin EA, Lee M, Jung KJ, Doyle MR, Amasino RM, Noh YS

Abstract

Flowering in Arabidopsis thaliana is controlled by multiple pathways, including the photoperiod pathway and the FLOWERING LOCUS C (FLC)-dependent pathway. Here, we report that a pair of related jumonji-class transcription factors, EARLY FLOWERING 6 (ELF6) and RELATIVE OF EARLY FLOWERING 6 (REF6), play divergent roles in the regulation of Arabidopsis flowering. ELF6 acts as a repressor in the photoperiod pathway, whereas REF6, which has the highest similarity to ELF6, is an FLC repressor. Ectopic expression studies and expression pattern analyses show that ELF6 and REF6 have different cellular roles and are also regulated differentially despite their sequence similarities. Repression of FLC expression by REF6 accompanies histone modifications in FLC chromatin, indicating that the transcriptional regulatory activity of this class of proteins includes chromatin remodeling. This report demonstrates the in vivo functions of this class of proteins in higher eukaryotes.

MeSH Terms
Amino Acid Sequence Arabidopsis/physiology Arabidopsis Proteins/chemistry,genetics,physiology Base Sequence DNA Primers Molecular Sequence Data Mutation Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Transcription Factors/chemistry,genetics,physiology Transcription, Genetic/genetics
Chemicals
Arabidopsis Proteins DNA Primers Transcription Factors
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Noh Bosl
Kumho Life and Environmental Science Laboratory, Puk-Gu, Kwangju 500-712, Korea. noh@kkpc.com <noh@kkpc.com>
Lee Seung-Hee
Kim Hyun-Jin
Yi Gibum
Shin Eun-Ah
Lee Mirha
Jung Kyung-Ja
Doyle Mark R
Amasino Richard M
Noh Yoo-Sun
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.104.025353
Email
noh@kkpc.com
Published
2004-10-00
Epub
2004-00-17
Pages
2601-13
Language
English
Region
England
NLM ID
9208688
PMCID
PMC520958
Subset
IM
Databases
GENBANK
AY664499, AY664500
External Links
PubMed source DOI: 10.1105/tpc.104.025353 PMC Full Text View journal details
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