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PMID: 15371339 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Two modes of DNA double-strand break repair are reciprocally regulated through the fission yeast cell cycle.

Genes & development ·Vol. 18 ·No. 18 ·2004-09-15 ·Pages 2249-54

Ferreira MG, Cooper JP

Abstract

Several considerations suggest that levels of the two major modes of double-strand break (DSB) repair, homologous recombination (HR), and nonhomologous end joining (NHEJ), are regulated through the cell cycle. However, this idea has not been explicitly tested. In the absence of the telomere-binding protein Taz1, fission yeast undergo lethal telomere fusions via NHEJ. These fusions occur only during periods of nitrogen starvation and fail to accumulate during logarithmic growth, when the majority of cells are in G2. We show that G1 arrest is the specific nitrogen starvation-induced event that promotes NHEJ between taz1(-) telomeres. Furthermore, the general levels of NHEJ and HR are reciprocally regulated through the cell cycle, so that NHEJ is 10-fold higher in early G1 than in other cell cycle stages; the reverse is true for HR. Whereas NHEJ is known to be dispensable for survival of DSBs in cycling cells, we find that it is critical for repair and survival of DSBs arising during G1.

MeSH Terms
Cell Cycle/genetics Cell Division DNA/genetics,metabolism DNA Repair/physiology G1 Phase/genetics,radiation effects G2 Phase/genetics Gamma Rays Nitrogen/metabolism Recombination, Genetic Schizosaccharomyces/genetics,radiation effects Schizosaccharomyces pombe Proteins/genetics,metabolism Telomere/genetics Telomere-Binding Proteins/genetics,metabolism
Chemicals
Schizosaccharomyces pombe Proteins Telomere-Binding Proteins taz1 protein, S pombe DNA Nitrogen
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ferreira Miguel Godinho
Telomere Biology Laboratory, Cancer Research UK, London WC2A 3PX, UK.
Cooper Julia Promisel
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.315804
Published
2004-09-15
Pages
2249-54
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC517518
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.315804 PMC Full Text View journal details
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