Differentiation of mouse embryonic stem cells into a defined neuronal lineage.

Animals Bicuculline/pharmacology Blotting, Western Calmodulin-Binding Proteins/metabolism Carrier Proteins/metabolism Cell Count/methods Cell Differentiation/drug effects,physiology,radiation effects Cell Lineage/physiology Cells, Cultured Drug Interactions Electric Stimulation/methods Embryo, Mammalian/cytology Excitatory Amino Acid Agonists/pharmacology Excitatory Amino Acid Antagonists/pharmacology Eye Proteins Fatty Acid-Binding Protein 7 Fatty Acid-Binding Proteins GABA Antagonists/pharmacology Glial Fibrillary Acidic Protein/metabolism Glutamic Acid/pharmacology Homeodomain Proteins/metabolism Immunohistochemistry/methods Indoles Intermediate Filament Proteins/metabolism Membrane Potentials/drug effects,radiation effects Mice Nerve Tissue Proteins/metabolism Nestin Neuroglia/metabolism Neurogranin Neurons/classification,cytology,drug effects,metabolism PAX6 Transcription Factor Paired Box Transcription Factors Patch-Clamp Techniques Quinoxalines/pharmacology Receptor, Nerve Growth Factor Receptor, trkA/metabolism Receptors, AMPA/metabolism Receptors, Nerve Growth Factor/metabolism Repressor Proteins Stem Cells/drug effects,physiology,radiation effects Synaptophysin Tetrodotoxin/pharmacology Time Factors Tretinoin/pharmacology Tubulin/metabolism Valine/analogs & derivatives,pharmacology

Calmodulin-Binding Proteins Carrier Proteins Excitatory Amino Acid Agonists Excitatory Amino Acid Antagonists Eye Proteins Fabp7 protein, mouse Fatty Acid-Binding Protein 7 Fatty Acid-Binding Proteins GABA Antagonists Glial Fibrillary Acidic Protein Homeodomain Proteins Indoles Intermediate Filament Proteins Nerve Tissue Proteins Nes protein, mouse Nestin Nrgn protein, mouse PAX6 Transcription Factor Paired Box Transcription Factors Pax6 protein, mouse Quinoxalines Receptor, Nerve Growth Factor Receptors, AMPA Receptors, Nerve Growth Factor Repressor Proteins Synaptophysin Tubulin beta3 tubulin, mouse 2,3-dioxo-6-nitro-7-sulfamoylbenzo(f)quinoxaline Neurogranin Glutamic Acid Tetrodotoxin DAPI Tretinoin 2-amino-5-phosphopentanoic acid Receptor, trkA Valine glutamate receptor ionotropic, AMPA 1 Bicuculline