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PMID: 1533042 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Dorsal-ventral patterning in Drosophila: DNA binding of snail protein to the single-minded gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 8 ·1992-04-15 ·Pages 3414-8

Kasai Y, Nambu JR, Lieberman PM, Crews ST

Abstract

The Drosophila snail gene is required for proper mesodermal development. Genetic studies suggest that it functions by repressing adjacent ectodermal gene expression including that of the single-minded (sim) gene. The snail gene encodes a protein with a zinc-finger motif, and here we report that the snail gene product is a sequence-specific DNA binding protein. The snail protein recognizes a 14-base-pair consensus sequence that is found nine times in a 2.8-kilobase sim regulatory region. These results provide evidence for the direct control of sim transcription by snail.

Related Genes
Sna sim
MeSH Terms
Adenovirus Early Proteins Alleles Animals Base Sequence Blastoderm/physiology DNA-Binding Proteins/genetics,metabolism Drosophila/embryology,genetics Embryo, Nonmammalian/physiology Genes Molecular Sequence Data Oligodeoxyribonucleotides Oncogene Proteins, Viral/genetics Promoter Regions, Genetic Zinc Fingers/genetics beta-Galactosidase/genetics,metabolism
Chemicals
Adenovirus Early Proteins DNA-Binding Proteins Oligodeoxyribonucleotides Oncogene Proteins, Viral beta-Galactosidase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kasai Y
Department of Biology, University of California, Los Angeles 90024.
Nambu J R
Lieberman P M
Crews S T
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29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.8.3414
Published
1992-04-15
Pages
3414-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC48878
Subset
IM
Grants
NICHD NIH HHS · R01 HD25251 · United States
NCI NIH HHS · T 32 CA09056 · United States
External Links
PubMed source DOI: 10.1073/pnas.89.8.3414 PMC Full Text View journal details
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